Yangxueqingnao particles inhibit rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid

• Published in: Journal of Zhejiang University. B. Science 2005-09, Vol.6 (9), p.892-896
• Main Author: 蔡巍 许毅 陈君柱 黄淑如 卢震亚 王战坤
• Format: Article
• Language: English
• Subjects: Animals; Biomedicine; Cell Proliferation - drug effects; Drugs, Chinese Herbal - pharmacology; Extracellular Signal-Regulated MAP Kinases - metabolism; Lysophospholipids - pharmacology; Male; Malondialdehyde - metabolism; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Rats; Rats, Sprague-Dawley; 中药制剂; 养血清脑颗粒; 细胞增生; 血管平滑肌细胞
• ISSN: 1673-1581; 1009-3095; 1862-1783
• DOI: 10.1631/jzus.2005.b0892

Objective： To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell （VSMC） proliferation induced by lysophosphatidic acid （LPA）. Methods： The amount of ^3H-TdR （^3H-thymidine） admixed in cultured rat VSMC was measured and mitogen-activated protein kinase （MAPK） activity and lipid peroxidation end product malondialdehyde （MDA） content of the VSMC were assayed. Results： 1×10^-9, 1×10^-8, 1×10^-7mol/L LPA in a concentration dependent manner, induced the amount of ^3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% （P〈0.01） respectively in the amount of 3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% （P〈0.05）, 29.6% （P〈0.01）, and 48.9% （P〈0.01） respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% （P〈0.01） respectively, in the 1×10^-7mol/L LPA-treated VSMC. Conclusions： LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.