Evaluation of ST13 gene expression in colorectal cancer patients

• Published in: Journal of Zhejiang University. B. Science 2005-12, Vol.6 (12), p.1170-1175
• Main Authors: Dong, Qing-hua, Zheng, Shu, Hu, Yue, Chen, Gong-xing, Ding, Jia-Yi
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - metabolism; Biomedicine; Carrier Proteins - metabolism; China - epidemiology; Colorectal cancer; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - mortality; Disease-Free Survival; Female; Gene expression; Gene Expression Profiling; Humans; Male; Oncology; Prevalence; Prognosis; Risk Assessment - methods; Risk Factors; ST13; Survival Analysis; Survival Rate; Tumor Suppressor Proteins - metabolism; 基因表达; 治疗方法; 结肠癌; 肿瘤组织
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.2005.B1170

We identified a novel gene ST13 from a subtraetive eDNA library of normal intestinal mueosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa （P〈0.05）. But there were no significant differences in the expression of ST13 as compared depth, lymph node metastasis and disease-specific survival. with different Dukes＇ stage, tumor differentiation grade, invasion