Intestinal microflora in rats with ischemia／reperfusion liver injury

• Published in: Journal of Zhejiang University. B. Science 2005-01, Vol.6 (1), p.14-21
• Main Author: 邢卉春 李兰娟 徐凯进 沈恬 陈云波 盛吉芳 俞云松 陈亚岗
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacterial Translocation; Biomedicine; Endotoxemia; Endotoxins; Endotoxins - blood; Enzymes; Histology; Injuries; Intestinal microflora; Intestinal Mucosa - microbiology; Intestine; Intestine, Small - microbiology; Ischemia; Kidneys; Liver; Liver - blood supply; Liver - injuries; Liver - metabolism; Liver - microbiology; Lymph nodes; Male; Malondialdehyde - metabolism; Mucosa; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - complications; Reperfusion Injury - metabolism; Reperfusion Injury - microbiology; Rodents; Specific pathogen free; Superoxide dismutase; Superoxide Dismutase - metabolism; Translocation; 内毒素; 局部缺血／多次灌注液; 细菌迁移; 肝损伤; 肠道微生物群
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.2005.B0014

Objectives: To investigate the intestinal microflora status related to ischemia/reperfusion (I/R) liver injury and explore the possible mechanism. Methods: Specific pathogen free grade Sprague-Dawley rats were randomized into three groups: Control group (n=8), sham group (n=6) and I/R group (n=10). Rats in the control group did not receive any treatment, rats in the I/R group were subjected to 20 rain of liver ischemia, and rats in the sham group were only subjected to sham operation. Twenty-two hours later, the rats were sacrificed and liver enzymes and malondialdehyde (MDA), superoxide dismutase (SOD), serum endotoxin,intestinal bacterial counts, intestinal mucosal histology, bacterial translocation to mesenteric lymph nodes, liver, spleen, and kidney were studied. Results: Ischemia/reperfusion increased liver enzymes, MDA, decreased SOD, and was associated with plasma endotoxin elevation in the I/R group campared to those in the sham group. Intestinal Bifidobacteria and Lactobacilli decreased and intestinal Enterobacterium and Enterococcus, bacterial translocation to kidney increased in the I/R group compared to the sham group. Intestinal microvilli were lost, disrupted and the interspace between cells became wider in the I/R group.Conclusion: I/R liver injury may lead to disturbance of intestinal microflora and impairment of intestinal mucosal barrier function,which contributes to endotoxemia and bacterial translocation to kidney.