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Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome
1. Blood platelets from normal children and children with the trisomy 21 form of Down's syndrome (mongolism) were studied to determine the cause of the well established reduction in platelet 5-HT in the disease. 2. Concentrations of endogenous 5-HT in the platelets from mongols were 25·3% of t...
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| Published in: | The Journal of physiology 1971-01, Vol.212 (2), p.287-297 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | 297 |
| container_issue | 2 |
| container_start_page | 287 |
| container_title | The Journal of physiology |
| container_volume | 212 |
| creator | Boullin, D. J. O'Brien, R. A. |
| description | 1. Blood platelets from normal children and children with the trisomy 21 form of Down's syndrome (mongolism) were studied
to determine the cause of the well established reduction in platelet 5-HT in the disease.
2. Concentrations of endogenous 5-HT in the platelets from mongols were 25·3% of the concentrations found in normal children.
3. The net accumulation of 5-HT in the mongol cells was decreased to 52·7% of normal. This reduction was probably due, in
part, to a defect in 5-HT transport, because the initial rates of 5-HT uptake at plasma concentrations of 10 -6 and 10 -5 M were significantly slower.
4. Experiments on the efflux of 5-HT from mongol platelets loaded with amine showed that the rate of loss was initially 2·6
and later 7·8 times faster than normal.
5. Platelet ATP in mongol cells was 26% of normal, and the reduction of ATP and 5-HT was in the molar ratio of 3:1.
6. It is considered that the low platelet 5-HT in Down's syndrome is due to a defective 5-HT transport mechanism and impaired
5-HT binding, resulting from a reduction in the essential binding substance, ATP. |
| doi_str_mv | 10.1113/jphysiol.1971.sp009325 |
| format | article |
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to determine the cause of the well established reduction in platelet 5-HT in the disease.
2. Concentrations of endogenous 5-HT in the platelets from mongols were 25·3% of the concentrations found in normal children.
3. The net accumulation of 5-HT in the mongol cells was decreased to 52·7% of normal. This reduction was probably due, in
part, to a defect in 5-HT transport, because the initial rates of 5-HT uptake at plasma concentrations of 10 -6 and 10 -5 M were significantly slower.
4. Experiments on the efflux of 5-HT from mongol platelets loaded with amine showed that the rate of loss was initially 2·6
and later 7·8 times faster than normal.
5. Platelet ATP in mongol cells was 26% of normal, and the reduction of ATP and 5-HT was in the molar ratio of 3:1.
6. It is considered that the low platelet 5-HT in Down's syndrome is due to a defective 5-HT transport mechanism and impaired
5-HT binding, resulting from a reduction in the essential binding substance, ATP.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1971.sp009325</identifier><identifier>PMID: 4251289</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Adenosine Triphosphate - metabolism ; Adolescent ; Binding Sites ; Biological Transport ; Blood Platelet Disorders - metabolism ; Blood Platelets - metabolism ; Child ; Child, Preschool ; Down Syndrome - metabolism ; Female ; Humans ; Male ; Serotonin - blood ; Serotonin - metabolism</subject><ispartof>The Journal of physiology, 1971-01, Vol.212 (2), p.287-297</ispartof><rights>1971 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5007-1676e169cef080d7745da0102f4ac9e1a4894fc088428629edb8181e17748b3a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1395660/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1395660/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4251289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boullin, D. J.</creatorcontrib><creatorcontrib>O'Brien, R. A.</creatorcontrib><title>Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Blood platelets from normal children and children with the trisomy 21 form of Down's syndrome (mongolism) were studied
to determine the cause of the well established reduction in platelet 5-HT in the disease.
2. Concentrations of endogenous 5-HT in the platelets from mongols were 25·3% of the concentrations found in normal children.
3. The net accumulation of 5-HT in the mongol cells was decreased to 52·7% of normal. This reduction was probably due, in
part, to a defect in 5-HT transport, because the initial rates of 5-HT uptake at plasma concentrations of 10 -6 and 10 -5 M were significantly slower.
4. Experiments on the efflux of 5-HT from mongol platelets loaded with amine showed that the rate of loss was initially 2·6
and later 7·8 times faster than normal.
5. Platelet ATP in mongol cells was 26% of normal, and the reduction of ATP and 5-HT was in the molar ratio of 3:1.
6. It is considered that the low platelet 5-HT in Down's syndrome is due to a defective 5-HT transport mechanism and impaired
5-HT binding, resulting from a reduction in the essential binding substance, ATP.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adolescent</subject><subject>Binding Sites</subject><subject>Biological Transport</subject><subject>Blood Platelet Disorders - metabolism</subject><subject>Blood Platelets - metabolism</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Down Syndrome - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Serotonin - blood</subject><subject>Serotonin - metabolism</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><recordid>eNqNkUtv1DAUhS0EKkPhJ4C8oqsMvs7L3iC15a1KsChry0luJi6OHewMQ_49HmVawQ55YcvnnO9aPoS8ArYFgPzN3TQs0Xi7BVnDNk6MyZyXj8gGikpmdS3zx2TDGOdZXpfwlDyL8Y4xyJmUZ-Ss4CVwITdkvGycD6O2ZjYYqe9pmQ1LF_zvZQ7LNOvROKT7dPiBVLuONsZ1xu1os9DGet_RyeoZLc6R9sGPtB2M7QI6ejDzQN_5g7uINC4uIUd8Tp702kZ8cdrPyfcP72-vP2U3Xz9-vr68ydqSsTqDqq4QKtlizwTr6rooO82A8b7QrUTQhZBF3zIhCi4qLrFrBAhASE7R5Do_J29X7rRvRuxadHPQVk3BjDosymuj_lWcGdTO_1KQy7KqWAK8PgGC_7nHOKvRxBat1Q79Pipx_G0OMhmr1dgGH2PA_mEIMHUsSt0XpY5FqfuiUvDl3098iJ2aSfrVqh-MxeU_qer2y7fjBQfOuagT5GKFDGY3HExAtcaibw3Oi0o-lVZy_gEqrLeB</recordid><startdate>19710101</startdate><enddate>19710101</enddate><creator>Boullin, D. J.</creator><creator>O'Brien, R. A.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19710101</creationdate><title>Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome</title><author>Boullin, D. J. ; O'Brien, R. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5007-1676e169cef080d7745da0102f4ac9e1a4894fc088428629edb8181e17748b3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Adolescent</topic><topic>Binding Sites</topic><topic>Biological Transport</topic><topic>Blood Platelet Disorders - metabolism</topic><topic>Blood Platelets - metabolism</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Down Syndrome - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Serotonin - blood</topic><topic>Serotonin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boullin, D. J.</creatorcontrib><creatorcontrib>O'Brien, R. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boullin, D. J.</au><au>O'Brien, R. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1971-01-01</date><risdate>1971</risdate><volume>212</volume><issue>2</issue><spage>287</spage><epage>297</epage><pages>287-297</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. Blood platelets from normal children and children with the trisomy 21 form of Down's syndrome (mongolism) were studied
to determine the cause of the well established reduction in platelet 5-HT in the disease.
2. Concentrations of endogenous 5-HT in the platelets from mongols were 25·3% of the concentrations found in normal children.
3. The net accumulation of 5-HT in the mongol cells was decreased to 52·7% of normal. This reduction was probably due, in
part, to a defect in 5-HT transport, because the initial rates of 5-HT uptake at plasma concentrations of 10 -6 and 10 -5 M were significantly slower.
4. Experiments on the efflux of 5-HT from mongol platelets loaded with amine showed that the rate of loss was initially 2·6
and later 7·8 times faster than normal.
5. Platelet ATP in mongol cells was 26% of normal, and the reduction of ATP and 5-HT was in the molar ratio of 3:1.
6. It is considered that the low platelet 5-HT in Down's syndrome is due to a defective 5-HT transport mechanism and impaired
5-HT binding, resulting from a reduction in the essential binding substance, ATP.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>4251289</pmid><doi>10.1113/jphysiol.1971.sp009325</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3751 |
| ispartof | The Journal of physiology, 1971-01, Vol.212 (2), p.287-297 |
| issn | 0022-3751 1469-7793 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1395660 |
| source | PubMed (Medline) |
| subjects | Adenosine Triphosphate - metabolism Adolescent Binding Sites Biological Transport Blood Platelet Disorders - metabolism Blood Platelets - metabolism Child Child, Preschool Down Syndrome - metabolism Female Humans Male Serotonin - blood Serotonin - metabolism |
| title | Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome |
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