Microarray analysis of fiber cell maturation in the lens

The mammalian lens consists of an aged core of quiescent cells enveloped by layers of mature fully elongated cells and younger, continuously elongating transcriptionally active cells. The fiber cell maturation is initiated when fiber cells cease to elongate. The process of maturation represents a ra...

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Bibliographic Details
Published in:FEBS letters 2005-02, Vol.579 (5), p.1213-1219
Main Authors: Ivanov, Dmitry, Dvoriantchikova, Galina, Pestova, Anna, Nathanson, Lubov, Shestopalov, Valery I.
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Differentiation
Gene expression profiling
laser capture microdisection
Laser capture microdissection
LCM
Lens
Lens, Crystalline - cytology
Lens, Crystalline - metabolism
Mice
Microarray
Oligonucleotide Array Sequence Analysis
Reproducibility of Results
RNA amplification
RNA, Messenger - analysis
RNA, Messenger - genetics
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Summary:The mammalian lens consists of an aged core of quiescent cells enveloped by layers of mature fully elongated cells and younger, continuously elongating transcriptionally active cells. The fiber cell maturation is initiated when fiber cells cease to elongate. The process of maturation represents a radical switch from active elongation to a life-long quiescence and has not been studied previously. It may also include critical stages of preparation for the organelle removal and denucleation. In the present study, we used laser capture microdisection (LCM) microdissection and RNA amplification to compare global gene expression profiles of young elongating and mature, non-elongating fiber cells. Analysis of microarray data from three independent dye-swap experiments identified 65 differentially expressed genes (FDR
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.01.016