Electrohydrodynamic jetting of mouse neuronal cells

CAD (Cath.a-differentiated) cells, a mouse neuronal cell line, were subjected to electrohydrodynamic jetting at a field strength of 0.47-0.67 kV/mm, corresponding to an applied voltage of 7-10 kV. After jetting, the cells appeared normal and continued to divide at rates similar to those shown by con...

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Bibliographic Details
Published in:Biochemical journal 2006-03, Vol.394 (Pt 2), p.375-378
Main Authors: Eagles, Peter A M, Qureshi, Amer N, Jayasinghe, Suwan N
Format: Article
Language:English
Subjects:
Accelerated Publication
Animals
Biotechnology - instrumentation
Biotechnology - methods
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Cell Survival
Electrochemistry - instrumentation
Electrochemistry - methods
Mice
Models, Biological
Neurons - cytology
Tissue Engineering - instrumentation
Tissue Engineering - methods
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Summary:CAD (Cath.a-differentiated) cells, a mouse neuronal cell line, were subjected to electrohydrodynamic jetting at a field strength of 0.47-0.67 kV/mm, corresponding to an applied voltage of 7-10 kV. After jetting, the cells appeared normal and continued to divide at rates similar to those shown by control samples. Jetted cells, when placed in serum-free medium, underwent differentiation that was sustained for at least 1 month. Some of the droplets produced by jetting contained only a few cells. These results indicate that the process of jetting does not significantly perturb neuronal cells and that this novel approach might in the future be a useful way to deposit small numbers of living nerve cells on to surfaces.
ISSN:0264-6021
1470-8728
DOI:10.1042/BJ20051838