Effects of solvent on the maximum charge state and charge state distribution of protein ions produced by electrospray ionization

The effects of solvent composition on both the maximum charge states and charge state distributions of analyte ions formed by electrospray ionization were investigated using a quadrupole mass spectrometer. The charge state distributions of cytochrome c and myoglobin, formed from 47%/50%/3% water/sol...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2000-11, Vol.11 (11), p.976-985
Main Authors: Iavarone, Anthony T, Jurchen, John C, Williams, Evan R
Format: Article
Language:English
Subjects:
Acetic acid
Acetonitrile
Algorithms
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Charge distribution
Charge transfer
Composition effects
Cytochrome
Cytochrome c Group - chemistry
Diethylamines - chemistry
Electrospraying
Ethylene glycol
Fundamental and applied biological sciences. Psychology
Hemoproteins
Hydrogen-Ion Concentration
Ionization
Ions
Mass spectrometry
Metalloproteins
Methyl cellosolve
Myoglobin - chemistry
Myoglobins
Peptides
Peptides - chemistry
Performance enhancement
Physical properties
Proteins
Proteins - chemistry
Quadrupoles
Solvents
Spectrometry, Mass, Electrospray Ionization - methods
Volatilization
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Summary:The effects of solvent composition on both the maximum charge states and charge state distributions of analyte ions formed by electrospray ionization were investigated using a quadrupole mass spectrometer. The charge state distributions of cytochrome c and myoglobin, formed from 47%/50%/3% water/solvent/acetic acid solutions, shift to lower charge (higher m/z) when the 50% solvent fraction is changed from water to methanol, to acetonitrile, to isopropanol. This is also the order of increasing gas-phase basicities of these solvents, although other physical properties of these solvents may also play a role. The effect is relatively small for these solvents, possibly due to their limited concentration inside the electrospray interface. In contrast, the addition of even small amounts of diethylamine (
ISSN:1044-0305
1879-1123
DOI:10.1016/S1044-0305(00)00169-0