Supercharged Protein and Peptide Ions Formed by Electrospray Ionization

The multiple charging of large molecules in electrospray ionization provides key advantages for obtaining accurate molecular weights by mass spectrometry and for obtaining structural information by tandem mass spectrometry and MS n experiments. Addition of glycerol or m-nitrobenzyl alcohol into the...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-04, Vol.73 (7), p.1455-1460
Main Authors: Iavarone, Anthony T, Jurchen, John C, Williams, Evan R
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Benzyl Alcohols - chemistry
Biological and medical sciences
Chemical reactions
Chemistry
Cytochrome c Group - chemistry
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Glycerol - chemistry
Ions
Myoglobin - chemistry
Peptides
Peptides - chemistry
Proteins
Proteins - chemistry
Scientific imaging
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:The multiple charging of large molecules in electrospray ionization provides key advantages for obtaining accurate molecular weights by mass spectrometry and for obtaining structural information by tandem mass spectrometry and MS n experiments. Addition of glycerol or m-nitrobenzyl alcohol into the electrospray solutions dramatically increases both the maximum observed charge state and the abundances of the high charge states of protein and peptide ions. Adding glycerol to acidified aqueous solutions of cytochrome c shifts the most abundant charge state from 17+ to 21+, shifts the maximum charge state from 20+ to 23+, and shifts the average charge state from 16.6+ to 20.9+. Much less m-nitrobenzyl alcohol (
ISSN:0003-2700
1520-6882
DOI:10.1021/ac001251t