Expression of HLA class II-associated peptide transporter and proteasome genes in human placentas and trophoblast cell lines

Expression of HLA class I antigens is closely controlled in the placental trophoblast cells, which interface directly with maternal cells during pregnancy. In this study, the possibility that peptide transporter (TAP-1, TAP-2) or proteasome (LMP7) genes might be involved in regulating antigen expres...

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Bibliographic Details
Published in:Immunology 1994-11, Vol.83 (3), p.444-448
Main Authors: Roby, K F, Fei, K, Yang, Y, Hunt, J S
Format: Article
Language:English
Subjects:
ATP-Binding Cassette Sub-Family B Member 2
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette, Sub-Family B, Member 3
Biological Transport
Blotting, Northern
Carrier Proteins - genetics
Cell Line
Cysteine Endopeptidases
Female
Gene Expression
Genes, MHC Class II
Humans
Multienzyme Complexes
Placenta - metabolism
Pregnancy
Pregnancy Trimester, First
Pregnancy Trimester, Third
Proteasome Endopeptidase Complex
Proteins - genetics
RNA, Messenger - analysis
Trophoblasts - metabolism
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Summary:Expression of HLA class I antigens is closely controlled in the placental trophoblast cells, which interface directly with maternal cells during pregnancy. In this study, the possibility that peptide transporter (TAP-1, TAP-2) or proteasome (LMP7) genes might be involved in regulating antigen expression in these or other cells that comprise placentas was investigated. Analysis by Northern blot hybridization showed that transcripts from all three genes were present in samples of first trimester and term placental RNA. TAP-1 and TAP-2 messages were consistently more abundant in early than in late gestation placentas, whereas the reverse was observed for LMP7 mRNA. Futher experiments were done on two trophoblast cell lines. One line, Jar, is negative for HLA class I, and the second, JEG-3, expresses HLA-G as well as other HLA class I genes. Both Jar and JEG-3 cells contained TAP-1, TAP-2 and LMP7 mRNA. With the exception of LMP7 in JEG-3 cells, message from all three genes was increased by treating the trophoblast cells with interferon-gamma. While no evidence was collected to support the postulate that the HLA class I negative status of some trophoblast cell subpopulations could be related to absent or dysfunctional TAP-1, TAP-2 or LMP7 mRNA, the data are consistent with the postulate that placental cell expression of HLA class I antigens could be influenced by the availability of peptide transporters and proteasome components.
ISSN:0019-2805
1365-2567