Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65

We have previously shown that mice vaccinated by injection with J774 macrophage-like tumour cells that expressed Mycobacterium leprae heat-shock protein (hsp) 65 as a transgene had acquired a remarkably high degree of protection against subsequent challenge with virulent M. tuberculosis. We show her...

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Bibliographic Details
Published in:Immunology 1994-11, Vol.83 (3), p.341-346
Main Authors: Silva, C L, Silva, M F, Pietro, R C, Lowrie, D B
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Antigen-Presenting Cells - immunology
Antigens, Bacterial - administration & dosage
Bacterial Proteins
CD8-Positive T-Lymphocytes - immunology
Chaperonin 60
Chaperonins - immunology
Heat-Shock Proteins - immunology
Immunization, Passive
Mice
Mice, Inbred BALB C
Mycobacterium leprae
Mycobacterium leprae - immunology
Mycobacterium tuberculosis
Receptors, Antigen, T-Cell, alpha-beta - immunology
Receptors, Antigen, T-Cell, gamma-delta - immunology
T-Lymphocytes - immunology
Tuberculosis - prevention & control
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Summary:We have previously shown that mice vaccinated by injection with J774 macrophage-like tumour cells that expressed Mycobacterium leprae heat-shock protein (hsp) 65 as a transgene had acquired a remarkably high degree of protection against subsequent challenge with virulent M. tuberculosis. We show here that antigen-specific T cells cloned from spleens of such vaccinated animals can transfer a high level of protection to non-vaccinated recipients. The most efficient cells were of T-cell receptor (TCR) alpha beta+ and CD4- CD8+ type and specifically lysed mycobacteria-infected macrophages. These findings are consistent with the importance for protective immunity of engaging the endogenous antigen-presenting pathway to bias the immune response towards a cytolytic action against a mycobacterial antigen that is expressed at the surface of infected macrophages. TCR gamma delta+ and TCR alpha beta+ cells interacted synergistically.
ISSN:0019-2805
1365-2567