Superantigen-induced peripheral T-cell deletion: the effects of chemical modification of antigen-presenting cells, interleukin-4 and glucocorticoid hormones

Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (GCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-l...

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Bibliographic Details
Published in:Immunology 1995-04, Vol.84 (4), p.528-535
Main Authors: Ayroldi, E, Cannarile, L, D'Adamio, F, Riccardi, C
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - drug effects
Antigen-Presenting Cells - immunology
Apoptosis - drug effects
Apoptosis - immunology
CD28 Antigens - immunology
Cell Division - immunology
Cells, Cultured
Dendritic Cells - immunology
Dexamethasone - pharmacology
Enterotoxins - immunology
Ethyldimethylaminopropyl Carbodiimide - pharmacology
Interleukin-4 - immunology
Mice
Mice, Inbred C3H
Superantigens - immunology
T-Lymphocyte Subsets - immunology
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Summary:Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (GCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-linker 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (ECDI) inhibited their capacity to induce SEB-specific deletion of mature T lymphocytes. Deletion was not influenced by treatment with anti-CD28 antibodies, which modulate T-cell activation. However, it was augmented by IL-4, known to counteract anti-CD3- and GCH-induced thymocyte apoptosis, and was inhibited by dexamethasone (DEX). These results indicate that metabolically active APC are required for deletion of antigen-specific mature T cells and suggest that IL-4 and GCH can modulate this phenomenon in vitro.
ISSN:0019-2805
1365-2567