Cell-surface marker analysis of rat thymic dendritic cells

Rat thymic dendritic cells have been isolated by collagenase digestion, separation of the low-density cell fraction by centrifugation on metrizamide, and differential adherence. The resulting dendritic cell preparation had a purity of > 90%, and has been analysed by flow cytometry (FCM) using a l...

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Bibliographic Details
Published in:Immunology 1993-06, Vol.79 (2), p.298-304
Main Authors: BANULS, M. P, ALVAREZ, A, FERRERO, I, ZAPATA, A, ARDAVIN, C
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibodies, Monoclonal - immunology
Antigens, CD - analysis
Antigens, Differentiation, T-Lymphocyte - analysis
Antigens, Surface - analysis
Biological and medical sciences
Cell Adhesion Molecules - analysis
Dendritic Cells - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Antigens - analysis
Immunobiology
Membrane Glycoproteins - analysis
Organs and cells involved in the immune response
Rats
Rats, Wistar
Thy-1 Antigens
Thymus Gland - immunology
Tumor Necrosis Factor Receptor Superfamily, Member 7
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Summary:Rat thymic dendritic cells have been isolated by collagenase digestion, separation of the low-density cell fraction by centrifugation on metrizamide, and differential adherence. The resulting dendritic cell preparation had a purity of > 90%, and has been analysed by flow cytometry (FCM) using a large panel of monoclonal antibodies (mAb). Dendritic cells expressed major histocompatibility (MHC) class I and class II molecules, the leucocyte common antigen CD45, the rat leucocyte antigen OX44, the rat macrophage marker ED1, and the adhesion molecules Mac-1, LFA-1 and ICAM-1. They were negative for the T- and B-cell-specific forms of CD45, CD45R and B220, and the B-cell marker OX12. Concerning T-cell marker expression, they were negative for T-cell receptor (TcR) and OX40, but they expressed CD2, CD4 and CD8, and interestingly, 50% of DC were CD5+, 50% expressed the alpha-chain of interleukin-2 receptor (IL-2R), and 80% were positive for the T-cell activation antigen recognized by the mAb OX48. Moreover, 60% of DC expressed high levels of Thy-1, whereas 40% displayed intermediate levels of this T-cell marker.
ISSN:0019-2805
1365-2567