Bacteriocin detection from whole bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Class I bacteriocins (lantibiotics) and class II bacteriocins are antimicrobial peptides secreted by gram-positive bacteria. Using two lantibiotics, lacticin 481 and nisin, and the class II bacteriocin coagulin, we showed that bacteriocins can be detected without any purification from whole producer...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology 2003-02, Vol.69 (2), p.1051-1058
Main Authors: Hindre, T, Didelot, S, Le Pennec, J.P, Haras, D, Dufour, A, Vallee-Rehel, K
Format: Article
Language:English
Subjects:
antibacterial properties
Bacillus - growth & development
Bacillus - metabolism
Bacillus coagulans
Bacteria
Bacterial Proteins - metabolism
bacteriocins
Bacteriocins - metabolism
bioassays
Biological and medical sciences
biosynthesis
chemical analysis
coagulin
Culture Media
Escherichia coli
Fundamental and applied biological sciences. Psychology
ionization
lacticin 481
Lactococcus lactis
Lactococcus lactis - growth & development
Lactococcus lactis - metabolism
lasers
mass spectrometry
Methods
Microbiology
nisin
Nisin - metabolism
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Thromboplastin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Class I bacteriocins (lantibiotics) and class II bacteriocins are antimicrobial peptides secreted by gram-positive bacteria. Using two lantibiotics, lacticin 481 and nisin, and the class II bacteriocin coagulin, we showed that bacteriocins can be detected without any purification from whole producer bacteria grown on plates by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). When we compared the results of MALDI-TOF-MS performed with samples of whole cells and with samples of crude supernatants of liquid cultures, the former samples led to more efficient bacteriocin detection and required less handling. Nisin and lacticin 481 were both detected from a mixture of their producer strains, but such a mixture can yield additional signals. We used this method to determine the masses of two lacticin 481 variants, which confirmed at the peptide level the effect of mutations in the corresponding structural gene.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.69.2.1051-1058.2003