The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco

MYB-related transcription factors are known to regulate different branches of flavonoid metabolism in plants and are believed to play wider roles in the regulation of phenylpropanoid metabolism in general. Here, we demonstrate that overexpression of two MYB genes from Antirrhinum represses phenolic...

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Bibliographic Details
Published in:The Plant cell 1998-02, Vol.10 (2), p.135-154
Main Authors: Tamagnone, L. (John Innes Centre, Norwich, UK.), Merida, A, Parr, A, MacKay, S, Culianez-Macia, F.A, Roberts, K, Martin, C
Format: Article
Language:English
Subjects:
4-COUMAROYL-COA LIGASE
ANTIRRHINUM
ANTIRRHINUM MAJUS
Biosynthesis
CAULIFLOWER MOSAIC CAULIMOVIRUS
Cell walls
CHEMICAL COMPOSITION
CHEMISTRY
CINNAMATE 4-HYDROXYLASE
COLOUR
COMPLEMENTARY DNA
DNA
ENZYMIC ACTIVITY
GENBANK/Y15607
GENE EXPRESSION
GENE OVEREXPRESSION
GENES
GENETIC TRANSFORMATION
HISTOCHEMISTRY
LEAVES
LIGASES
Lignin
LIGNINS
MESSENGER RNA
MOLECULAR SEQUENCE DATA
NECROSIS
NICOTIANA TABACUM
NUCLEOTIDE SEQUENCE
OXIDOREDUCTASES
OXYGENASES
PHENOLIC COMPOUNDS
Phenotypes
PHENYLALANINE AMMONIA LYASE
PLANT ANATOMY
Plant cells
Plants
PROMOTERS
RECOMBINANT DNA
SEGREGATION
STRUCTURAL GENES
TRANSCRIPTION FACTORS
TRANSGENIC PLANTS
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Summary:MYB-related transcription factors are known to regulate different branches of flavonoid metabolism in plants and are believed to play wider roles in the regulation of phenylpropanoid metabolism in general. Here, we demonstrate that overexpression of two MYB genes from Antirrhinum represses phenolic acid metabolism and lignin biosynthesis in transgenic tobacco plants. The inhibition of this branch of phenylpropanoid metabolism appears to be specific to AmMYB308 and AmMYB330, suggesting that they recognize their normal target genes in these transgenic plants. Experiments with yeast indicate that AmMYB308 can act as a very weak transcriptional activator so that overexpression may competitively inhibit the activity of stronger activators recognizing the same target motifs. The effects of the transcription factors on inhibition of phenolic acid metabolism resulted in complex modifications of the growth and development of the transgenic plants. The inhibition of monolignol production resulted in plants with at least 17% less lignin in their vascular tissue. This reduction is of importance when designing strategies for the genetic modification of woody crops
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.10.2.135