Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis

In yeast, the DMC1 gene is required for interhomolog recombination, which is an essential step for bivalent formation and the correct partition of chromosomes during meiosis I. By using a reverse genetics approach, we were able to identify a T-DNA insertion in AtDMC1, the Arabidopsis homolog of DMC1...

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Bibliographic Details
Published in:The Plant cell 1999-09, Vol.11 (9), p.1623-1634
Main Authors: Couteau, F, Belzile, F, Horlow, C, Grandjean, O, Vezon, D, Doutriaux, M.P
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases
Animals
anthers
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins
Arabidopsis thaliana
Base Sequence
callose
Cell Cycle Proteins
chromosome disposition
chromosome segregation
Chromosomes
Chromosomes - genetics
DNA
DNA Primers - genetics
DNA-Binding Proteins - genetics
fertility
gametogenesis
Genes, Plant
genetic complementation
Genetic Complementation Test
Genetics
histochemistry
insertional mutagenesis
insertional mutations
Life Sciences
megaspore mother cells
Megasporocytes
Meiosis
Meiosis - genetics
Mice
Mother cells
Mutagenesis, Insertional
mutants
Mutation
Nuclear Proteins
ova
Ovules
Phenotype
Plant cells
Plant Proteins - genetics
Plants
Plants genetics
Pollen
pollen mother cells
pollen tubes
Rec A Recombinases
sporulation
transposons
Yeasts
Citations: Items that cite this one
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Description
Summary:In yeast, the DMC1 gene is required for interhomolog recombination, which is an essential step for bivalent formation and the correct partition of chromosomes during meiosis I. By using a reverse genetics approach, we were able to identify a T-DNA insertion in AtDMC1, the Arabidopsis homolog of DMC1. Homozygotes for the AtDMC1 insertion failed to express AtDMC1, and their residual fertility was 1.5% that of the wild type. Complete fertility was restored in mutant plants when a wild-type copy of the AtDMC1 gene was reintroduced. Cytogenetical analysis points to a correlation of the sterility phenotype with severely disturbed chromosome behavior during both male and female meiosis. In this study, our data demonstrate that AtDMC1 function is crucial for meiosis in Arabidopsis. However, meiosis can be completed in the Arabidopsis dmc1 mutant, which is not the case for mouse or some yeast mutants.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.11.9.1623