Requirements for growth of Epstein-Barr virus-transformed cells at low cell densities

We investigated the requirements for growth of Epstein-Barr virus-transformed cells at low cell densities in a homotypic system: only Epstein-Barr virus-transformed cells or their products were used to supply feeder activity. The cloning efficiency was increased markedly under conditions favouring c...

Full description

Saved in:
Bibliographic Details
Published in:Immunology 1987-02, Vol.60 (2), p.187-193
Main Authors: TIEBOUT, R. F, SAUERWEIN, R. W, VAN DER MEER, W. G. J, VAN BOXTEL-OOSTERHOF, F, ZEIJLEMAKER, W. P
Format: Article
Language:English
Subjects:
Antibody Formation
B-Lymphocytes - metabolism
Biological and medical sciences
Cell Communication
Cell Division - drug effects
Cell Line
Cell Transformation, Viral
Culture Media
Epstein-Barr virus
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Herpesvirus 4, Human
Humans
Immunobiology
Immunological reactions in vitro
Leukocyte Count
Lymphoid cell lines. Hybrids
Tetradecanoylphorbol Acetate - pharmacology
Thymidine - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated the requirements for growth of Epstein-Barr virus-transformed cells at low cell densities in a homotypic system: only Epstein-Barr virus-transformed cells or their products were used to supply feeder activity. The cloning efficiency was increased markedly under conditions favouring close cell-to-cell contact: culture in round-bottomed rather than in flat-bottomed wells or the addition of irradiated Epstein-Barr virus-transformed cells. Further enhancement was obtained by the addition of the tumour promoter phorbol-myristate acetate. Part of this effect was also induced by supernatants of Epstein-Barr virus-transformed cells, in particular when the latter had been stimulated with phorbol ester. Thus, under limiting dilution conditions, Epstein-Barr virus-transformed cells were found to be dependent upon autologous cell-mediated and soluble proliferation-stimulating signals. In such a homotypic feeder system, the cloning efficiency could be enhanced up to eight-fold; a 100% cloning efficiency could not be achieved. Evidence is presented that the residual deficit in cloning efficiency is inherent to these cell lines.
ISSN:0019-2805
1365-2567