The immune response of guinea-pigs to type II collagen: poor cross-reactivity with homologous type II collagen accounts for resistance to collagen-induced arthritis

In order to determine the susceptibility of guinea-pigs to collagen-induced arthritis (CIA), Hartley and Strain 13 guinea-pigs were immunized with heterologous or homologous type II collagen. None of the animals developed CIA. Because immunity to type II collagen plays a critical role in CIA, we cha...

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Bibliographic Details
Published in:Immunology 1988-04, Vol.63 (4), p.619-624
Main Authors: HERNANDEZ, A. D, CREMER, M. A, TOWNES, A. S, STUART, J. M, KANG, A. H
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibody production
Arthritis - immunology
Biological and medical sciences
Collagen - administration & dosage
Collagen - immunology
Cross Reactions
Disease Susceptibility
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Guinea Pigs
Hypersensitivity, Delayed - immunology
Immunity, Cellular
Immunobiology
Immunoglobulin G - biosynthesis
Injections, Intra-Articular
Male
Mice
Rats
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Summary:In order to determine the susceptibility of guinea-pigs to collagen-induced arthritis (CIA), Hartley and Strain 13 guinea-pigs were immunized with heterologous or homologous type II collagen. None of the animals developed CIA. Because immunity to type II collagen plays a critical role in CIA, we characterized the guinea-pig's immune response to determine the basis for this resistance. Guinea-pigs develop cellular and humoral reactivity to heterologous type II collagen similar to that of CIA-susceptible rats. The reactions distinguish type I from type II collagen but not among several heterologous type II collagens. The cell-mediated immune (CMI) response was specific for determinants on the primary amino acid structure of collagen, whether native or denatured collagen was used for immunization; however, the humoral response was specific for the form of the molecule used for immunization. Guinea-pigs differ from CIA-susceptible rats in that immunization with homologous or heterologous type II collagen fails to induce significant cross-reactive immunity with the homologous antigen. A transient arthritis could be induced in animals immunized with heterologous type II collagen by injecting them intra-articularly with heterologous but not with homologous type II collagen. Our results show that the disparity between immunity to type II collagen and the susceptibility to develop CIA in guinea-pigs is due to their poor cross-reactive immune response to autologous type II collagen.
ISSN:0019-2805
1365-2567