Complement titration by rectilinear attenuance and attempts at its automatization

Immune haemolysis was determined by rectilinear attenuance at 625 mμ ( l = 1.3 mm, 1.25 mm), and the data obtained treated by von Krogh's empirical equation. The C′H 50 /ml values obtained by the attenuance method had high correlation with those obtained by the conventional photometric method (...

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Bibliographic Details
Published in:Immunology 1969-12, Vol.17 (6), p.831-845
Main Authors: Sato, P S, Yoshida, T O, Fukusima, Y, Ono, N, Yoshida, K, Ito, Y
Format: Article
Language:English
Subjects:
Animals
Autoanalysis
Complement Fixation Tests
Complement System Proteins - analysis
Erythrocytes
Guinea Pigs
Hemoglobins - analysis
Hemolysis
Methods
Sheep
Spectrophotometry
Time Factors
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Summary:Immune haemolysis was determined by rectilinear attenuance at 625 mμ ( l = 1.3 mm, 1.25 mm), and the data obtained treated by von Krogh's empirical equation. The C′H 50 /ml values obtained by the attenuance method had high correlation with those obtained by the conventional photometric method (541 mμ). Automatic complement titration was attempted, as a part of the basic studies on quantitative complement fixation tests.
ISSN:0019-2805
1365-2567