The [URE3] prion is not conserved among Saccharomyces species

The [URE3] prion of Saccharomyces cerevisiae is a self-propagating inactive form of the nitrogen catabolism regulator Ure2p. To determine whether the [URE3] prion is conserved in S. cerevisiae-related yeast species, we have developed genetic tools allowing the detection of [URE3] in Saccharomyces pa...

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Published in:Genetics (Austin) 2005-09, Vol.171 (1), p.23-34
Main Authors: Talarek, Nicolas, Maillet, Laurent, Cullin, Christophe, Aigle, Michel
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biochemistry, Molecular Biology
Conserved Sequence - genetics
Evolution, Molecular
Evolutionary biology
Genetic Complementation Test
Genetics
Glutathione Peroxidase
Investigations
Life Sciences
Molecular biology
Molecular Sequence Data
Phylogeny
Prions
Prions - genetics
Prions - metabolism
Proteins
Saccharomyces
Saccharomyces - genetics
Saccharomyces - growth & development
Saccharomyces - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces paradoxus
Saccharomyces uvarum
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Yeast
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description The [URE3] prion of Saccharomyces cerevisiae is a self-propagating inactive form of the nitrogen catabolism regulator Ure2p. To determine whether the [URE3] prion is conserved in S. cerevisiae-related yeast species, we have developed genetic tools allowing the detection of [URE3] in Saccharomyces paradoxus and Saccharomyces uvarum. We found that [URE3] is conserved in S. uvarum. In contrast, [URE3] was not detected in S. paradoxus. The inability of S. paradoxus Ure2p to switch to a prion isoform results from the primary sequence of the protein and not from the lack of cellular cofactors as heterologous Ure2p can propagate [URE3] in this species. Our data therefore demonstrate that [URE3] is conserved only in a subset of Saccharomyces species. Implications of our finding on the physiological and evolutionary meaning of the yeast [URE3] prion are discussed.
doi_str_mv 10.1534/genetics.105.043489
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source Freely Accessible Science Journals - check A-Z of ejournals; Oxford Journals Online; Alma/SFX Local Collection
subjects Amino Acid Sequence
Biochemistry, Molecular Biology
Conserved Sequence - genetics
Evolution, Molecular
Evolutionary biology
Genetic Complementation Test
Genetics
Glutathione Peroxidase
Investigations
Life Sciences
Molecular biology
Molecular Sequence Data
Phylogeny
Prions
Prions - genetics
Prions - metabolism
Proteins
Saccharomyces
Saccharomyces - genetics
Saccharomyces - growth & development
Saccharomyces - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces paradoxus
Saccharomyces uvarum
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Yeast
title The [URE3] prion is not conserved among Saccharomyces species
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