CBT1 Interacts Genetically With CBP1 and the Mitochondrially Encoded Cytochrome b Gene and Is Required to Stabilize the Mature Cytochrome b mRNA of Saccharomyces cerevisiae

Mutation of a CCG sequence in the 5'-untranslated region of the mitochondrially encoded cytochrome b mRNA in Saccharomyces cerevisiae results in destabilization of the message and respiratory deficiency of the mutant strain. This phenotype mimics that of a mutation in the nuclear CBP1 gene. Her...

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Bibliographic Details
Published in:Genetics (Austin) 2005-11, Vol.171 (3), p.949-957
Main Authors: Ellis, Timothy P, Schonauer, Melissa S, Dieckmann, Carol L
Format: Article
Language:English
Subjects:
Cytochromes b - genetics
Cytochromes b - metabolism
DNA polymerase
Genes
Investigations
Microbiology
Mitochondria - genetics
Mitochondrial DNA
Mitochondrial Proteins
Mutation
Point Mutation
Retroelements - physiology
RNA Stability - genetics
RNA, Fungal - genetics
RNA, Fungal - metabolism
RNA, Messenger - metabolism
RNA, Ribosomal - metabolism
RNA-Binding Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
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Summary:Mutation of a CCG sequence in the 5'-untranslated region of the mitochondrially encoded cytochrome b mRNA in Saccharomyces cerevisiae results in destabilization of the message and respiratory deficiency of the mutant strain. This phenotype mimics that of a mutation in the nuclear CBP1 gene. Here it is shown that overexpression of the nuclear CBT1 gene, due to a transposon insertion in the 5'-untranslated region, rescues the respiratory defects resulting from mutating the CCG sequence to ACG. Overexpressing alleles of CBT1 are allelic to soc1, a previously isolated suppressor of cbp1ts-induced temperature sensitivity of respiratory growth. Quantitative primer extension analysis indicated that cbt1 null strains have defects in 5'-end processing of precursor cytochrome b mRNA to the mature form. Cbt1p is also required for stabilizing the mature cytochrome b mRNA after 5' processing.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.104.036467