Immunological senescence. II. Normal in vitro colony formation by B cells from old mice

The in vitro frequency and proliferative capacity of B cells and B-cell precursors in old mice was assessed. The mitogenic response to the B-cell mitogens lipopolysaccharide (LPS) and dextran sulphate decline later and at a slower rate than the T-cell response to phytohaemagglutinin and concanavalin...

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Bibliographic Details
Published in:Immunology 1979-06, Vol.37 (2), p.293-299
Main Authors: Duwe, A K, Roder, J C, Singhal, S K
Format: Article
Language:English
Subjects:
Aging
Animals
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
Bone Marrow Cells
Clone Cells
Colony-Forming Units Assay
Leukocyte Count
Mice
Mice, Inbred Strains
Mitogens - pharmacology
Mitosis - drug effects
Spleen - cytology
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
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Summary:The in vitro frequency and proliferative capacity of B cells and B-cell precursors in old mice was assessed. The mitogenic response to the B-cell mitogens lipopolysaccharide (LPS) and dextran sulphate decline later and at a slower rate than the T-cell response to phytohaemagglutinin and concanavalin A. The response to precursor B-cell mitogen dextran sulphate was not depressed in the bone marrow of aging mice. In addition, the dose response and kinetics of LPS and mercaptoethanol stimulated B-cell colony formation in agar was identical in spleen cells from young and old mice. These results indicate that the intrinsic proliferative capacity of B cells from old mice is normal.
ISSN:0019-2805
1365-2567