A transgene encoding a plasma membrane H+-ATPase that confers acid resistance in Arabidopsis thaliana seedlings

Proton pumps (H+-ATPases) are the primary active transport systems in the plasma membrane of higher plant cells. These enzymes are encoded by a large gene family expressed throughout the plant, with specific isoforms directed to various specialized cells. While their involvement in membrane energeti...

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Bibliographic Details
Published in:Genetics (Austin) 1998-06, Vol.149 (2), p.501-507
Main Authors: Young, J.C, DeWitt, N.D, Sussman, M.R
Format: Article
Language:English
Subjects:
ACIDEZ
ACIDITE
ACIDITY
Acids - pharmacology
AHA3 GENE
Arabidopsis - drug effects
Arabidopsis - enzymology
Arabidopsis - genetics
ARABIDOPSIS THALIANA
Cell Membrane - drug effects
Cell Membrane - enzymology
Cell Membrane - genetics
CELL MEMBRANES
CHEMISTRY
CHIMIE
CRECIMIENTO
CROISSANCE
Drug Resistance - genetics
Enzymes
FENOTIPOS
FLOEMA
Flowers & plants
GENE
GENES
Genes, Plant
Genetics
GROWTH
H+-TRANSPORTING ATPASE
HIDROLASAS
HIPOCOTILOS
HYDROLASE
HYDROLASES
HYPOCOTYL ELONGATION
HYPOCOTYLE
HYPOCOTYLS
IMMUNOCYTOCHEMISTRY
IMMUNOHISTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Membranes
MUTANT
MUTANTES
MUTANTS
PHENOTYPE
PHENOTYPES
PHLOEM
PHLOEME
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants - enzymology
Plants - genetics
PLANTULAS
PLANTULE
PYROPHOSPHATASES
QUIMICA
SEEDLINGS
SEGREGACION
SEGREGATION
Signal Transduction - drug effects
Signal Transduction - genetics
Space life sciences
STRUCTURAL GENES
Transgenes - genetics
TRANSGENIC PLANTS
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Summary:Proton pumps (H+-ATPases) are the primary active transport systems in the plasma membrane of higher plant cells. These enzymes are encoded by a large gene family expressed throughout the plant, with specific isoforms directed to various specialized cells. While their involvement in membrane energetics has been suggested by a large body of biochemical and physiological studies, a genetic analysis of their role in plants has not yet been performed. We report here that mutant Arabidopsis thaliana plants containing a phloem-specific transgene encoding a plasma membrane H+-ATPase with an altered carboxy terminus show improved growth at low pH during seedling development. These observations provide the first genetic evidence for a role of the plasma membrane H+-ATPase in cytoplasmic pH homeostasis in plants.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/149.2.501