Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae

The calcium-dependent protein phosphatase calcineurin plays an essential role in ion homeostasis in yeast. In this study, we identify a parallel ion stress response pathway that is independent of the calcineurin signaling pathway. Cells with null alleles in both STD1 and its homologue, MTH1, manifes...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 1998-09, Vol.150 (1), p.31-42
Main Authors: Ganster, R.W. (University of Pittsburgh, PA.), McCartney, R.R, Schmidt, M.C
Format: Article
Language:English
Subjects:
Adaptation, Physiological - genetics
ALPHA FACTOR
ANIONS
Base Sequence
BINDING PROTEINS
Calcineurin - metabolism
CATIONS
CHLORIDES
CYTOTOXINS
DNA Primers
ESTERASES
Fungal Proteins - genetics
Fungal Proteins - metabolism
GENE DOSAGE
GENE EXPRESSION
Gene Expression Regulation, Fungal
GENE INTERACTION
GENES
GENETIC REGULATION
GENETICS
GLUCOSE
Glucose - metabolism
HAL1 GENE
Homeostasis
Ions
LITHIUM
LITHIUM CHLORIDE
MESSENGER RNA
MUTANTS
Mutation
MYCOTOXINS
Oxidative Stress
Phenotype
PHENOTYPES
PHOSPHOPROTEIN PHOSPHATASE
PMR2 GENE
POTASSIUM CHLORIDE
PROTEIN KINASE
Proteins
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - physiology
SALINITY
SALT TOLERANCE
Signal Transduction
Sodium - metabolism
SODIUM CHLORIDE
STD1 GENE
STRESS
STRUCTURAL GENES
TOXINS
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The calcium-dependent protein phosphatase calcineurin plays an essential role in ion homeostasis in yeast. In this study, we identify a parallel ion stress response pathway that is independent of the calcineurin signaling pathway. Cells with null alleles in both STD1 and its homologue, MTH1, manifest numerous phenotypes observed in calcineurin mutants, including sodium, lithium, manganese, and hydroxyl ion sensitivity, as well as alpha factor toxicity. Furthermore, increased gene dosage of STD1 suppresses the ion stress phenotypes in calcineurin mutants and confers halotolerance in wild-type cells. However, Std1p functions in a calcineurin-independent ion stress response pathway, since a std1 mth1 mutant is FK506 sensitive under conditions of ion stress. Mutations in other genes known to regulate gene expression in response to changes in glucose concentration, including SNF3, RGT2, and SNF5, also affect cell growth under ion stress conditions. Gene expression studies indicate that the regulation of HAL1 and PMR2 expression is affected by STD1 gene dosage. Taken together, our data demonstrate that response to ion stress requires the participation of both calcineurin-dependent and -independent pathways.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/150.1.31