Escherichia coli MutM Suppresses Illegitimate Recombination Induced by Oxidative Stress

DNA damage by oxidative stress is one of the causes of mutagenesis. However, whether or not DNA damage induces illegitimate recombination has not been determined. To study the effect of oxidative stress on illegitimate recombination, we examined the frequency of lambdabio transducing phage in the pr...

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Bibliographic Details
Published in:Genetics (Austin) 1999-02, Vol.151 (2), p.439-446
Main Authors: Onda, Masaaki, Hanada, Katsuhiro, Kawachi, Hirokazu, Ikeda, Hideo
Format: Article
Language:English
Subjects:
Bacteria
Bacteriophage lambda - genetics
Deoxyribonucleic acid
DNA
DNA Repair
DNA-Formamidopyrimidine Glycosylase
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins
Genes, Bacterial
Genetics
Mutation
N-Glycosyl Hydrolases - genetics
Oxidative Stress - genetics
Phage lambda
Proteins
Recombination, Genetic
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Summary:DNA damage by oxidative stress is one of the causes of mutagenesis. However, whether or not DNA damage induces illegitimate recombination has not been determined. To study the effect of oxidative stress on illegitimate recombination, we examined the frequency of lambdabio transducing phage in the presence of hydrogen peroxide and found that this reagent enhances illegitimate recombination. To clarify the types of illegitimate recombination, we examined the effect of mutations in mutM and related genes on the process. The frequency of lambdabio transducing phage was 5- to 12-fold higher in the mutM mutant than in the wild type, while the frequency in the mutY and mutT mutants was comparable to that of the wild type. Because 7,8-dihydro-8-oxoguanine (8-oxoG) and formamido pyrimidine (Fapy) lesions can be removed from DNA by MutM protein, these lesions are thought to induce illegitimate recombination. Analysis of recombination junctions showed that the recombination at Hotspot I accounts for 22 or 4% of total lambdabio transducing phages in the wild type or in the mutM mutant, respectively. The preferential increase of recombination at nonhotspot sites with hydrogen peroxide in the mutM mutant was discussed on the basis of a new model, in which 8-oxoG and/or Fapy residues may introduce double-strand breaks into DNA.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/151.2.439