Trichome cell growth in Arabidopsis thaliana can be derepressed by mutations in at least five genes

Leaf trichomes in Arabidopsis are unicellular epidermal hairs with a branched morphology. They undergo successive endoreduplication rounds early during cell morphogenesis. Mutations affecting trichome nuclear DNA content, such as triptychon or glabra3, alter trichome branching. We isolated new mutan...

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Bibliographic Details
Published in:Genetics (Austin) 1999-05, Vol.152 (1), p.461-476
Main Authors: Perazza, D, Herzog, M, Hulskamp, M, Brown, S, Dorne, A.M, Bonneville, J.M
Format: Article
Language:English
Subjects:
Alleles
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
branching
Cell Division - genetics
cell growth
Cells
Chromosome Mapping
cytochemistry
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - metabolism
DNA Mutational Analysis
Flow Cytometry
gene interaction
Genes
Genetic Complementation Test
Genetic Linkage
linkage (genetics)
Microscopy, Fluorescence
Models, Genetic
Mutagenesis
mutants
Mutation
Phenotype
Physical growth
polyploidy
Repressor Proteins - metabolism
trichomes
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Summary:Leaf trichomes in Arabidopsis are unicellular epidermal hairs with a branched morphology. They undergo successive endoreduplication rounds early during cell morphogenesis. Mutations affecting trichome nuclear DNA content, such as triptychon or glabra3, alter trichome branching. We isolated new mutants with supernumerary trichome branches, which fall into three unlinked complementation groups: KAKTUS and the novel loci, POLYCHOME and RASTAFARI. They map to chromosomes IV, II, and V, respectively. The trichomes of these mutants presented an increased DNA content, although to a variable extent. The spindly-5 mutant, which displays a constitutive gibberellin response, also produces overbranched trichomes containing more nuclear DNA. We analyzed genetic interactions using double mutants and propose that two independent pathways, defined by SPINDLY and TRIPTYCHON, act to limit trichome growth. KAKTUS and POLYCHOME might have redundant actions mediating gibberellin control via SPINDLY. The overall leaf polysomaty was not notably affected by these mutations, suggesting that they affect the control of DNA synthesis in a tissue- or cell type-specific manner. Wild-type tetraploids also produce overbranched trichomes; they displayed a shifted polysomaty in trichomes and in the whole leaf, suggesting a developmental program controlling DNA increases via the counting of endoreduplication rounds.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/152.1.461