Segregation distortion of T-DNA markers linked to the self-incompatibility (S) locus in Petunia hybrida

In plants with a gametophytic self-incompatibility system the specificity of the pollen is determined by the haploid genotype at the self-incompatibility (S) locus. In certain crosses this can lead to the exclusion of half the gametes from the male parent carrying a particular S-allele. This leads t...

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Bibliographic Details
Published in:Genetics (Austin) 2000-03, Vol.154 (3), p.1323-1333
Main Authors: Harbord, R M, Napoli, C A, Robbins, T P
Format: Article
Language:English
Subjects:
Crosses, Genetic
Deoxyribonucleic acid
DNA
DNA, Bacterial
DNA, Plant
Flowers & plants
Genetic Linkage
Genetic Markers
Meiosis
Petunia hybrida
Plant reproduction
S gene
Solanaceae - genetics
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Summary:In plants with a gametophytic self-incompatibility system the specificity of the pollen is determined by the haploid genotype at the self-incompatibility (S) locus. In certain crosses this can lead to the exclusion of half the gametes from the male parent carrying a particular S-allele. This leads to pronounced segregation distortion for any genetic markers that are linked to the S-locus. We have used this approach to identify T-DNA insertions carrying a maize transposable element that are linked to the S-locus of Petunia hybrida. A total of 83 T-DNA insertions were tested for segregation distortion of the selectable marker used during transformation with Agrobacterium. Segregation distortion was observed for 12 T-DNA insertions and at least 8 of these were shown to be in the same linkage group by intercrossing. This indicates that differential transmission of a single locus (S) is probably responsible for all of these examples of T-DNA segregation distortion. The identification of selectable markers in coupling with a functional S-allele will allow the preselection of recombination events around the S-locus in petunia. Our approach provides a general method for identifying transgenes that are linked to gametophytic self-incompatibility loci and provides an opportunity for transposon tagging of the petunia S-locus.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/154.3.1323