Strength of Translation Initiation Signal Sequence of mRNA as Studied by Quantification Method: Effect of Nucleotide Substitutions Upon Translation Efficiency in Rat Preproinsulin mRNA

Concerning the translation initiation signals in vertebrate mRNAs, both the ATG initiation codon and the sequences flanking the initiation codon are required to direct the position of initiation. A consensus sequence for the signal, (GCC)GCC(A or G)CCATGG, has been proposed, but actual initiation se...

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Bibliographic Details
Published in:Nucleic acids research 1996-09, Vol.24 (17), p.3313-3316
Main Authors: Iida, Yôichi, Masuda, Takeshi
Format: Article
Language:English
Subjects:
Animals
Codon, Initiator
Consensus Sequence
DNA Mutational Analysis
Insulin
Mutagenesis
Peptide Chain Initiation, Translational - genetics
Proinsulin - biosynthesis
Proinsulin - genetics
Protein Precursors - biosynthesis
Protein Precursors - genetics
Rats
RNA, Messenger - genetics
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Summary:Concerning the translation initiation signals in vertebrate mRNAs, both the ATG initiation codon and the sequences flanking the initiation codon are required to direct the position of initiation. A consensus sequence for the signal, (GCC)GCC(A or G)CCATGG, has been proposed, but actual initiation sequences differ from it to a greater or lesser degree. In the present report, the translation initiation signal sequences of rat preproinsulin and its mutant mRNAs were analyzed using a quantification method proposed previously. In this method, each 16 nt sequence in the mRNA was characterized by its sample score, which shows strength of the signal. So far, Kozak has constructed a number of preproinsulin mutant mRNAs in which nucleotides flanking the ATG codon are systematically varied, and measured the translation initiation efficiency in terms of the proinsulin product. Her experimental results were well understood on the basis of the strength of the translation initiation signal sequence.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/24.17.3313