A Simple Sequence Repeat-Based Linkage Map of Barley

A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of info...

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Bibliographic Details
Published in:Genetics (Austin) 2000-12, Vol.156 (4), p.1997-2005
Main Authors: Ramsay, L, Macaulay, M, degli Ivanissevich, S, MacLean, K, Cardle, L, Fuller, J, Edwards, K. J, Tuvesson, S, Morgante, M, Massari, A, Maestri, E, Marmiroli, N, Sjakste, T, Ganal, M, Powell, W, Waugh, R
Format: Article
Language:English
Subjects:
Analysis
Chromosome Mapping
Crosses, Genetic
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
Genes, Plant
Genetic Linkage
Genetic Markers
Genetics
Genome, Plant
Grain
Hordeum - genetics
Hordeum vulgare
Repetitive Sequences, Nucleic Acid
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Summary:A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F(1) of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci. Centromeric clustering of markers was observed in the main mapping population; however, the clustering severity was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/156.4.1997