Effect of the Suppressor of Underreplication (SuUR) Gene on Position-Effect Variegation Silencing in Drosophila melanogaster

It has been previously shown that the SuUR gene encodes a protein located in intercalary and pericentromeric heterochromatin in Drosophila melanogaster polytene chromosomes. The SuUR mutation suppresses the formation of ectopic contacts and DNA underreplication in polytene chromosomes; SuUR+ in extr...

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Bibliographic Details
Published in:Genetics (Austin) 2003-11, Vol.165 (3), p.1209-1220
Main Authors: Belyaeva, E. S, Boldyreva, L. V, Volkova, E. I, Nanayev, R. A, Alekseyenko, A. A, Zhimulev, I. F
Format: Article
Language:English
Subjects:
Animals
Deoxyribonucleic acid
DNA
DNA - biosynthesis
DNA - genetics
DNA Replication - genetics
DNA-Binding Proteins - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Female
Gene Dosage
Gene Silencing
Genetics
Male
Mutation
Proteins
Suppressor of Underreplication gene
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Summary:It has been previously shown that the SuUR gene encodes a protein located in intercalary and pericentromeric heterochromatin in Drosophila melanogaster polytene chromosomes. The SuUR mutation suppresses the formation of ectopic contacts and DNA underreplication in polytene chromosomes; SuUR+ in extra doses enhances the expression of these characters. This study demonstrates that heterochromatin-dependent PEV silencing is also influenced by SuUR. The SuUR protein localizes to chromosome regions compacted as a result of PEV; the SuUR mutation suppresses DNA underreplication arising in regions of polytene chromosomes undergoing PEV. The SuUR mutation also suppresses variegation of both adult morphological characters and chromatin compaction observed in rearranged chromosomes. In contrast, SuUR+ in extra doses and its overexpression enhance variegation. Thus, SuUR affects PEV silencing in a dose-dependent manner. However, its effect is expressed weaker than that of the strong modifier Su(var)2-5.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/165.3.1209