Molecular Haplotyping of Genetic Markers 10 kb Apart by Allele-Specific Long-Range PCR

Haplotypes, combinations of polymorphic markers in a chromosome, are critical for genome diversity research. However, their utility in population samplings is compromised by uncertain linkage phase determinations from unrelated individuals. Molecular haplotyping accomplishes direct phase determinati...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1996-12, Vol.24 (23), p.4841-4843
Main Authors: Michalatos-Beloin, Sonia, Tishkoff, Sarah A., Bentley, Kevin L., Kidd, Kenneth K., Ruano, Gualberto
Format: Article
Language:English
Subjects:
Alleles
CD4 Antigens - genetics
Gene Deletion
Genetic Linkage
Genetic Markers
Genotype
Haplotypes
Humans
Polymerase Chain Reaction - methods
Repetitive Sequences, Nucleic Acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Haplotypes, combinations of polymorphic markers in a chromosome, are critical for genome diversity research. However, their utility in population samplings is compromised by uncertain linkage phase determinations from unrelated individuals. Molecular haplotyping accomplishes direct phase determination by generation of hemizygous templates from diploid genomic samples. We report molecular haplotyping by allele-specific long-range PCR of two markers 9.5 kb apart at the CD4 locus: a bi-allelic Alu deletion and a multi-allelic repeat. We verified CD4 molecular haplotypes by classical Mendelian analysis. Molecular haplotyping should prove useful in mapping disease genes and in establishing founder effects.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/24.23.4841