Identification of S-Nitrosylation Motifs by Site-Specific Mapping of the S-Nitrosocysteine Proteome in Human Vascular Smooth Muscle Cells

• Published in: Proceedings of the National Academy of Sciences - PNAS 2006-05, Vol.103 (19), p.7420-7425
• Main Authors: Greco, Todd M., Hodara, Roberto, Parastatidis, Loannis, Heijnen, Harry F. G., Dennehy, Michelle K., Liebler, Daniel C., Ischiropoulos, Harry
• Format: Article
• Language: English
• Subjects: 14-3-3 proteins; Adducts; Amino Acid Sequence; Biological Sciences; Cells, Cultured; Circulatory system; Cysteine - analogs & derivatives; Cysteine - chemistry; Cysteine - metabolism; Humans; Mass Spectrometry; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Molecular Sequence Data; Muscle Cells - chemistry; Muscle Cells - metabolism; Muscle, Smooth, Vascular - chemistry; Muscle, Smooth, Vascular - metabolism; Muscular system; Nitric oxide; Nitrogen - chemistry; Nitrogen - metabolism; Oxides; Peptide elongation factors; Peptides - chemistry; Physiological regulation; Propylamines; Proteins; Proteome - chemistry; Proteome - metabolism; Proteomes; Proteomics; S-Nitrosothiols - chemistry; S-Nitrosothiols - metabolism; Sequence analysis; Signal transduction; Smooth muscle myocytes
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0600729103

S-nitrosylation, the selective modification of cysteine residues in proteins to form S-nitrosocysteine, is a major emerging mechanism by which nitric oxide acts as a signaling molecule. Even though nitric oxide is intimately involved in the regulation of vascular smooth muscle cell functions, the potential protein targets for nitric oxide modification as well as structural features that underlie the specificity of protein S-nitrosocysteine formation in these cells remain unknown. Therefore, we used a proteomic approach using selective peptide capturing and site-specific adduct mapping to identify the targets of S-nitrosylation in human aortic smooth muscle cells upon exposure to S-nitrosocysteine and propylamine propylamine NONOate. This strategy identified 20 unique S-nitrosocysteine-containing peptides belonging to 18 proteins including cytoskeletal proteins, chaperones, proteins of the translational machinery, vesicular transport, and signaling. Sequence analysis of the S-nitrosocysteine-containing peptides revealed the presence of acid/base motifs, as well as hydrophobic motifs surrounding the identified cysteine residues. High-resolution immunogold electron microscopy supported the cellular localization of several of these proteins. Interestingly, seven of the 18 proteins identified are localized within the ER/Golgi complex, suggesting a role for S-nitrosylation in membrane trafficking and ER stress response in vascular smooth muscle.