A homogeneous method to quantify mRNA levels: a hybridization of RNase protection and scintillation proximity assay technologies

A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a single reagent post RNase digestion. As a...

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Bibliographic Details
Published in:Nucleic acids research 1997-07, Vol.25 (14), p.2947-2948
Main Authors: Kenrick, M K, Jiang, L, Potts, C L, Owen, P J, Shuey, D J, Econome, J G, Anson, J G, Quinet, E M
Format: Article
Language:English
Subjects:
Animals
Apolipoprotein A-I - genetics
Nucleic Acid Hybridization - methods
Rats
Reproducibility of Results
Ribonucleases - metabolism
RNA, Messenger - analysis
Scintillation Counting
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Summary:A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a single reagent post RNase digestion. As a model system, the inducible expression of rat apolipoprotein-A1 mRNA has been measured by both traditional gel-based RPAs and the SPA-based RPA assay. Results demonstrate that the ribonuclease protection proximity assay (RiPPA) faithfully reproduces the gel-based results and is at least as sensitive as many existing methods.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.14.2947