The RNase P RNA from cyanobacteria: short tandemly repeated repetitive (STRR) sequences are present within the RNase P RNA gene in heterocyst-forming cyanobacteria

The RNase P RNA gene (rnpB) from 10 cyanobacteria has been characterized. These new RNAs, together with the previously available ones, provide a comprehensive data set of RNase P RNA from diverse cyanobacterial lineages. All heterocystous cyanobacteria, but none of the non-heterocystous strains anal...

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Bibliographic Details
Published in:Nucleic acids research 1997-09, Vol.25 (17), p.3471-3477
Main Author: Vioque, A
Format: Article
Language:English
Subjects:
Anabaena
Base Composition
Base Sequence
Cyanobacteria - enzymology
Cyanobacteria - genetics
Cyanophyta
Dermocarpa
Endoribonucleases - genetics
Fischerella
Molecular Sequence Data
Mutagenesis, Site-Directed
Nostoc
Nucleic Acid Conformation
Oscillatoria
Phylogeny
Repetitive Sequences, Nucleic Acid
Ribonuclease P
RNA, Bacterial - chemistry
RNA, Catalytic - genetics
Synechocystis
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Summary:The RNase P RNA gene (rnpB) from 10 cyanobacteria has been characterized. These new RNAs, together with the previously available ones, provide a comprehensive data set of RNase P RNA from diverse cyanobacterial lineages. All heterocystous cyanobacteria, but none of the non-heterocystous strains analyzed, contain short tandemly repeated repetitive (STRR) sequences that increase the length of helix P12. Site-directed mutagenesis experiments indicate that the STRR sequences are not required for catalytic activity in vitro. STRR sequences seem to have recently and independently invaded the RNase P RNA genes in heterocyst-forming cyanobacteria because closely related strains contain unrelated STRR sequences. Most cyanobacteria RNase P RNAs lack the sequence GGU in the loop connecting helices P15 and P16 that has been established to interact with the 3'-end CCA in precursor tRNA substrates in other bacteria. This character is shared with plastid RNase P RNA. Helix P6 is longer than usual in most cyanobacteria as well as in plastid RNase P RNA.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.17.3471