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Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture
Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served...
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Published in: | Environmental health perspectives 1997-09, Vol.105 (suppl 5), p.1031-1036 |
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creator | Mollo, Laura Levresse, Valerie Ottaviani, Maria F. Ellouk-Achard, Sophie Jaurand, Marie-Claude Fubini, Bice |
description | Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells. |
doi_str_mv | 10.1289/ehp.97105s51031 |
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Department of Health, Education and Welfare</publisher><subject>Animals ; Cell culture techniques ; Cell lines ; Cell membranes ; Cells, Cultured ; Cultured cells ; Electron paramagnetic resonance ; Electron Spin Resonance Spectroscopy ; Environmental health ; Epithelial Cells - metabolism ; Epithelium - chemistry ; Epithelium - metabolism ; Line spectra ; Materials ; Micelles ; Particle Size ; Physical and Chemical Properties of Particles/Mechanisms of Toxicity ; Pleura - cytology ; Pleura - metabolism ; Proteins - chemistry ; Rats ; Silicon Dioxide - chemistry ; Spectroscopic analysis ; Spin Labels ; Surface Properties</subject><ispartof>Environmental health perspectives, 1997-09, Vol.105 (suppl 5), p.1031-1036</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-78728698503491b7747a7014265f3ef678dd93407bbdce9792608cc7d142758c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3433504$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3433504$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772,58217,58450</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9400695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mollo, Laura</creatorcontrib><creatorcontrib>Levresse, Valerie</creatorcontrib><creatorcontrib>Ottaviani, Maria F.</creatorcontrib><creatorcontrib>Ellouk-Achard, Sophie</creatorcontrib><creatorcontrib>Jaurand, Marie-Claude</creatorcontrib><creatorcontrib>Fubini, Bice</creatorcontrib><title>Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture</title><title>Environmental health perspectives</title><addtitle>Environ Health Perspect</addtitle><description>Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells.</description><subject>Animals</subject><subject>Cell culture techniques</subject><subject>Cell lines</subject><subject>Cell membranes</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Electron paramagnetic resonance</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Environmental health</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium - chemistry</subject><subject>Epithelium - metabolism</subject><subject>Line spectra</subject><subject>Materials</subject><subject>Micelles</subject><subject>Particle Size</subject><subject>Physical and Chemical Properties of Particles/Mechanisms of Toxicity</subject><subject>Pleura - cytology</subject><subject>Pleura - metabolism</subject><subject>Proteins - chemistry</subject><subject>Rats</subject><subject>Silicon Dioxide - chemistry</subject><subject>Spectroscopic analysis</subject><subject>Spin Labels</subject><subject>Surface Properties</subject><issn>0091-6765</issn><issn>1552-9924</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpVkUtvEzEUhS1EVUJhzQrJC8Ru2uvxa7xBQhEvKagVgbXl8XgaF2ccbA-oa_54TRJVYWXJ57vnXPsg9IrAJWk7deU2u0slCfDMCVDyBC0I522jVMueogWAIo2Qgj9Dz3O-AwDSCXGOzhUDEIov0N91mYd7HEdcNg6vi-l98GV_YfCNSWZrbidXvMUr07uAV3766QZcIv7qctzFFOeM13XGGrye02isw37CyzgVYwv-48sGfzNlT9eE4E3ASxdC3lNzKHNyL9DZaEJ2L4_nBfrx8cP35edmdf3py_L9qrGMdaWRnWw7oToOlCnSS8mkkUBYK_hI3ShkNwyKMpB9P1inpGoFdNbKoSKSd5ZeoHcH393cb11lppJM0Lvktybd62i8_l-Z_Ebfxt-asJrDVTV4ezRI8dfsctFbn219jZlc_QdNBBNCSqjg1QG0Keac3PgYQkD_603X3vRJb3Xi9eluj_yxqKq_Oeh3ucR0atdSkJoySjkw-gDKaKCp</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Mollo, Laura</creator><creator>Levresse, Valerie</creator><creator>Ottaviani, Maria F.</creator><creator>Ellouk-Achard, Sophie</creator><creator>Jaurand, Marie-Claude</creator><creator>Fubini, Bice</creator><general>National Institute of Environmental Health Sciences. 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Department of Health, Education and Welfare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>19970901</creationdate><title>Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture</title><author>Mollo, Laura ; Levresse, Valerie ; Ottaviani, Maria F. ; Ellouk-Achard, Sophie ; Jaurand, Marie-Claude ; Fubini, Bice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-78728698503491b7747a7014265f3ef678dd93407bbdce9792608cc7d142758c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Cell culture techniques</topic><topic>Cell lines</topic><topic>Cell membranes</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>Electron paramagnetic resonance</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Environmental health</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium - chemistry</topic><topic>Epithelium - metabolism</topic><topic>Line spectra</topic><topic>Materials</topic><topic>Micelles</topic><topic>Particle Size</topic><topic>Physical and Chemical Properties of Particles/Mechanisms of Toxicity</topic><topic>Pleura - cytology</topic><topic>Pleura - metabolism</topic><topic>Proteins - chemistry</topic><topic>Rats</topic><topic>Silicon Dioxide - chemistry</topic><topic>Spectroscopic analysis</topic><topic>Spin Labels</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mollo, Laura</creatorcontrib><creatorcontrib>Levresse, Valerie</creatorcontrib><creatorcontrib>Ottaviani, Maria F.</creatorcontrib><creatorcontrib>Ellouk-Achard, Sophie</creatorcontrib><creatorcontrib>Jaurand, Marie-Claude</creatorcontrib><creatorcontrib>Fubini, Bice</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Environmental health perspectives</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mollo, Laura</au><au>Levresse, Valerie</au><au>Ottaviani, Maria F.</au><au>Ellouk-Achard, Sophie</au><au>Jaurand, Marie-Claude</au><au>Fubini, Bice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture</atitle><jtitle>Environmental health perspectives</jtitle><addtitle>Environ Health Perspect</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>105</volume><issue>suppl 5</issue><spage>1031</spage><epage>1036</epage><pages>1031-1036</pages><issn>0091-6765</issn><eissn>1552-9924</eissn><abstract>Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells.</abstract><cop>United States</cop><pub>National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare</pub><pmid>9400695</pmid><doi>10.1289/ehp.97105s51031</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cell culture techniques Cell lines Cell membranes Cells, Cultured Cultured cells Electron paramagnetic resonance Electron Spin Resonance Spectroscopy Environmental health Epithelial Cells - metabolism Epithelium - chemistry Epithelium - metabolism Line spectra Materials Micelles Particle Size Physical and Chemical Properties of Particles/Mechanisms of Toxicity Pleura - cytology Pleura - metabolism Proteins - chemistry Rats Silicon Dioxide - chemistry Spectroscopic analysis Spin Labels Surface Properties |
title | Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture |
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