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Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture

Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served...

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Published in:Environmental health perspectives 1997-09, Vol.105 (suppl 5), p.1031-1036
Main Authors: Mollo, Laura, Levresse, Valerie, Ottaviani, Maria F., Ellouk-Achard, Sophie, Jaurand, Marie-Claude, Fubini, Bice
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container_end_page 1036
container_issue suppl 5
container_start_page 1031
container_title Environmental health perspectives
container_volume 105
creator Mollo, Laura
Levresse, Valerie
Ottaviani, Maria F.
Ellouk-Achard, Sophie
Jaurand, Marie-Claude
Fubini, Bice
description Stable radicals detectable by electron paramagnetic resonance (EPR) may be of use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells.
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ispartof Environmental health perspectives, 1997-09, Vol.105 (suppl 5), p.1031-1036
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source PubMed Central(OpenAccess); JSTOR Archival Journals and Primary Sources Collection
subjects Animals
Cell culture techniques
Cell lines
Cell membranes
Cells, Cultured
Cultured cells
Electron paramagnetic resonance
Electron Spin Resonance Spectroscopy
Environmental health
Epithelial Cells - metabolism
Epithelium - chemistry
Epithelium - metabolism
Line spectra
Materials
Micelles
Particle Size
Physical and Chemical Properties of Particles/Mechanisms of Toxicity
Pleura - cytology
Pleura - metabolism
Proteins - chemistry
Rats
Silicon Dioxide - chemistry
Spectroscopic analysis
Spin Labels
Surface Properties
title Study of the Stability of a Paramagnetic Label Linked to Mesoporous Silica Surface in Contact with Rat Mesothelial Cells in Culture
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