One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors

In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.

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Bibliographic Details
Published in:Genetics (Austin) 2004, Vol.166 (1), p.631-635
Main Authors: Andrulis, E.D, Zappulla, D.C, Alexieva-Botcheva, K, Evangelista, C, Sternglanz, R
Format: Article
Language:English
Subjects:
Binding sites
Deoxyribonucleic acid
DNA
DNA-Binding Proteins
Gene Silencing
Genes
Genes, Fungal
Genetics
Hybridization
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics
Telomere - genetics
Transcription Factors - genetics
Two-Hybrid System Techniques
Yeast
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Description
Summary:In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.166.1.631