Repair of degraded duplex DNA from prehistoric samples using Escherichia coli DNA polymerase I and T4 DNA ligase

The most notable feature of DNA extracted from prehistoric material is that it is of poor quality. Amplification of PCR products from such DNA is consequently an exception. Here we present a simple method for the repair of degraded duplex DNA using the enzymes Escherichia coli DNA polymerase I and T...

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Bibliographic Details
Published in:Nucleic acids research 1998-02, Vol.26 (3), p.857-859
Main Authors: Pusch, Carsten M., Giddings, Ian, Scholz, Michael
Format: Article
Language:English
Subjects:
Antigens, Neoplasm
Base Sequence
Blood Proteins - genetics
DNA - chemistry
DNA - history
DNA Damage
DNA Ligases
DNA Polymerase I
DNA Repair
Escherichia coli - enzymology
Germany
Haptoglobins
History of medicine
History, Ancient
Humans
Molecular Sequence Data
Polymerase Chain Reaction - methods
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Summary:The most notable feature of DNA extracted from prehistoric material is that it is of poor quality. Amplification of PCR products from such DNA is consequently an exception. Here we present a simple method for the repair of degraded duplex DNA using the enzymes Escherichia coli DNA polymerase I and T4 DNA ligase. Adjacent sequences separated by nicks do not split up into intact strands during the denaturation step of PCR. Thus the target DNA is refractory to amplification. The proposed repair of nicked, fragmented ancient DNA results in an increase of amplification efficiency, such that the correct base order of the respective nuclear DNA segment can be obtained.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/26.3.857