Structure determination of lipid bilayers

A method of determining the phases of X-ray reflections from oriented model membrane systems at low resolution is described. The method involves deconvolution and requires that d less than or equal to 2v where v is the width of the head group region within the bilayer and d is the thickness of the b...

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Bibliographic Details
Published in:Biophysical journal 1978-09, Vol.23 (3), p.407-425
Main Authors: Worthington, C.R., Kharf, R.S.
Format: Article
Language:English
Subjects:
Hydrocarbons
Lipids
Membranes, Artificial
Phosphatidylcholines
Phosphatidylethanolamines
X-Ray Diffraction
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Summary:A method of determining the phases of X-ray reflections from oriented model membrane systems at low resolution is described. The method involves deconvolution and requires that d less than or equal to 2v where v is the width of the head group region within the bilayer and d is the thickness of the bilayer. The method can be used with a single set of X-ray data and applies to lipid bilayers which have a relatively constant density in the hydrocarbon region. Phases for the first five or six orders of phosphatidylethanolamine and lecithin are derived. A refined analysis based upon deconvolution but using information inherent in the Fourier profile is also described.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(78)85459-9