Cytoplasmic ribosomal protein genes of the fission yeast Schizosaccharomyces pombe display a unique promoter type: a suggestion for nomenclature of cytoplasmic ribosomal proteins in databases

We identified 34 new ribosomal protein genes in the Schizosaccharomyces pombe database at the Sanger Centre coding for 30 different ribosomal proteins. All contain the Homol D-box in their promoter. We have shown that Homol D is, in this promoter type, the TATA-analogue. Many promoters contain the H...

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Bibliographic Details
Published in:Nucleic acids research 1998-07, Vol.26 (14), p.3319-3322
Main Authors: Gross, T, Kaufer, N.F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
beta-galactosidase
Cytoplasm - metabolism
enzyme activity
genbank/aj002733
genbank/aj002734
gene expression
gene symbols
genes
homol d-box
Introns
Molecular Sequence Data
nucleotide sequences
Oligodeoxyribonucleotides
promoter regions
Promoter Regions, Genetic
proteins
recombinant DNA
reporter genes
Ribosomal Proteins - genetics
ribosomes
Schizosaccharomyces - genetics
Schizosaccharomyces pombe
Sequence Homology, Amino Acid
terminology
Terminology as Topic
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Summary:We identified 34 new ribosomal protein genes in the Schizosaccharomyces pombe database at the Sanger Centre coding for 30 different ribosomal proteins. All contain the Homol D-box in their promoter. We have shown that Homol D is, in this promoter type, the TATA-analogue. Many promoters contain the Homol E-box, which serves as a proximal activation sequence. Furthermore, comparative sequence analysis revealed a ribosomal protein gene encoding a protein which is the equivalent of the mammalian ribosomal protein L28. The budding yeast Saccharomyces cerevisiae has no L28 equivalent. Over the past 10 years we have isolated and characterized nine ribosomal protein (rp) genes from the fission yeast S.pombe. This endeavor yielded promoters which we have used to investigate the regulation of rp genes. Since eukaryotic ribosomal proteins are remarkably conserved and several rp genes of the budding yeast S.cerevisiae were sequenced in 1985, we probed DNA fragments encoding S.cerevisiae ribosomal proteins with genomic libraries of S.pombe. The deduced amino acid sequence of the different isolated rp genes of fission yeast share between 65 and 85% identical amino acids with their counterparts of budding yeast.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/26.14.3319