Application of Minimal Sequence Quality Values Prevents Misidentification of the blaSHV Type in Single Bacterial Isolates Carrying Different SHV Extended-Spectrum β-Lactamase Genes

Nucleotide sequencing is the standard molecular method for determination of the β-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of blaSHV genes, as illustrated by three strains of three different species that each contained two different blaSH...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-05, Vol.44 (5), p.1896-1898
Main Authors: al Naiemi, Nashwan, Schipper, Kim, Duim, Birgitta, Bart, Aldert
Format: Article
Language:English
Subjects:
Alleles
Bacteria - enzymology
Bacteria - genetics
Bacteria - isolation & purification
Bacteriology
Base Sequence
beta-Lactamases - genetics
Biological and medical sciences
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Enterobacter cloacae - enzymology
Enterobacter cloacae - genetics
Enterobacter cloacae - isolation & purification
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - isolation & purification
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Humans
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Polymerase Chain Reaction - methods
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - isolation & purification
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Description
Summary:Nucleotide sequencing is the standard molecular method for determination of the β-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of blaSHV genes, as illustrated by three strains of three different species that each contained two different blaSHV alleles, SHV-2 and SHV-12.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.44.5.1896-1898.2006