Development of a HPLC/Tandem-MS Method for the Analysis of the Larvicides Methoprene, Hydroprene, and Kinoprene at Trace Levels Using Diels−Alder Derivatization

The invasion and subsequent spread of the mosquito-borne West Nile virus in the United States has resulted in increased use of methoprene. With the increased need for sensitive detection and monitoring of methoprene in the environment, an analytical LC/ESI−MS/MS method has been developed for the ana...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-05, Vol.53 (9), p.3306-3312
Main Authors: Aronov, Pavel A, Dettmer, Katja, Christiansen, Julie A, Cornel, Anthony J, Hammock, Bruce D
Format: Article
Language:English
Subjects:
bioassays
Biological and medical sciences
chemical reactions
Chromatography, High Pressure Liquid - methods
Culicidae
Diels-Alder reaction
Fatty Acids, Unsaturated - analysis
flooded pastures
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
hydroprene
Indicators and Reagents
insecticidal properties
insecticide residues
insecticide resistance
Insecticides - analysis
juvenile hormone analogs
Juvenile Hormones
larvicides
mass spectrometry
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
methoprene
Methoprene - analysis
Ochlerotatus
Ochlerotatus nigromaculis
pastures
Pesticide Residues - analysis
Spectrometry, Mass, Electrospray Ionization - methods
surface water
Vectors. Intermediate hosts
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Summary:The invasion and subsequent spread of the mosquito-borne West Nile virus in the United States has resulted in increased use of methoprene. With the increased need for sensitive detection and monitoring of methoprene in the environment, an analytical LC/ESI−MS/MS method has been developed for the analysis of methoprene and two analogues, kinoprene and hydroprene, in water. To improve the ionization efficiency of the nonpolar analytes, a derivatization step with the Cookson-type reagent 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) was used. Derivatization improved the limit of detection 100-fold. For tandem MS analyses, limits of detection in environmental water samples (S/N = 3) are about 6 pg/mL for methoprene and 20 pg/mL for kinoprene and hydroprene, resulting in limits of quantification (S/N = 10) of 20 pg/mL for methoprene and 60 pg/mL for hydroprene and kinoprene extracted from 10 mL of water. This method was applied to measure methoprene concentrations in water samples from a treated site. Keywords: Methoprene; kinoprene; hydroprene; West Nile virus; ESI−MS; Cookson-type reagent
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0485842