Pogo transposase contains a putative helix-turn-helix DNA binding domain that recognises a 12 bp sequence within the terminal inverted repeats

Pogo is a transposable element with short terminal inverted repeats. It contains two open reading frames that are joined by splicing and code for the putative pogo transposase, the sequence of which indicates that it is related to the transposases of members of the Tc1/mariner family as well as prot...

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Bibliographic Details
Published in:Nucleic acids research 1999-01, Vol.27 (2), p.455-461
Main Authors: Wang, H, Hartswood, E, Finnegan, D.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
DNA Transposable Elements
DNA-binding domains
DNA-binding proteins
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drosophila melanogaster
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
Drosophila Proteins
Escherichia coli - genetics
Helix-Turn-Helix Motifs
Molecular Sequence Data
nucleotide sequences
Protein Binding
Protein Structure, Secondary
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - metabolism
repetitive sequences
Terminal Repeat Sequences
Transposases - chemistry
Transposases - genetics
Transposases - metabolism
transposons
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Summary:Pogo is a transposable element with short terminal inverted repeats. It contains two open reading frames that are joined by splicing and code for the putative pogo transposase, the sequence of which indicates that it is related to the transposases of members of the Tc1/mariner family as well as proteins that have no known transposase activity including the centromere binding protein CENP-B. We have shown that the N-terminal region of pogo transposase binds in a sequence-specific manner to the ends of pogo and have identified residues essential for this. The results are consistent with a prediction that DNA binding is due to a helix-turn-helix motif within this region. The transposase recognises a 12 bp sequence, two copies of which are present at each end of pogo DNA. The outer two copies occur as inverted repeats 14 nucleotides from each end of the element, and contain a single base mismatch and indicate the inverted repeats of pogo are 26 nucleotides long. The inner copies occur as direct repeats, also with a single mismatch.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/27.2.455