Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response

Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representi...

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Bibliographic Details
Published in:Infection and Immunity 2006-07, Vol.74 (7), p.3853-3863
Main Authors: Mukhopadhyay, Sanghamitra, Miller, Richard D, Sullivan, Erin D, Theodoropoulos, Christina, Mathews, Sarah A, Timms, Peter, Summersgill, James T
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Cell Line, Tumor
Chlamydophila pneumoniae
Chlamydophila pneumoniae - pathogenicity
Chlamydophila pneumoniae - physiology
Chlamydophila pneumoniae - ultrastructure
Fundamental and applied biological sciences. Psychology
Heat-Shock Proteins - biosynthesis
Hot Temperature
Humans
Interferon-gamma - physiology
Iron - metabolism
Microbiology
Miscellaneous
Models, Biological
Molecular Pathogenesis
Proteome - biosynthesis
Proteomics
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Summary:Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representing nine functional subgroups, from the gamma interferon (IFN-γ) treatment (primarily tryptophan limitation) and iron limitation (IL) models of persistence compared to those following heat shock (HS) at 42°C. Protein expression patterns of C. pneumoniae persistence indicates a strong stress component, as evidenced by the upregulation of proteins involved in protein folding, assembly, and modification. However, it is clearly more than just a stress response. In IFN persistence, but not IL or HS, amino acid and/or nucleotide biosynthesis proteins were found to be significantly upregulated. In contrast, proteins involved in the biosynthesis of cofactors, cellular processes, energy metabolism, transcription, and translation showed an increased in expression in only the IL model of persistence. These data represent the most extensive protein expression study of C. pneumoniae comparing the chlamydial heat shock stress response to two models of persistence and identifying the common and unique protein level responses during persistence.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.02104-05