Contrapuntal Networks of Gene Expression during Arabidopsis Seed Filling

We have used cDNA microarrays to examine changes in gene expression during Arabidopsis seed development and to compare wild-type and mutant wrinkled1 (wri1) seeds that have an 80% reduction in oil. Between 5 and 13 days after flowering, a period preceding and including the major accumulation of stor...

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Bibliographic Details
Published in:The Plant cell 2002-06, Vol.14 (6), p.1191-1206
Main Authors: Ruuska, Sari A., Girke, Thomas, Benning, Christoph, Ohlrogge, John B.
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - physiology
Biosynthesis
DNA, Complementary
Enzymes
Fatty acids
Fatty Acids - metabolism
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes
Genomics
Genomics Article
Messenger RNA
Oligonucleotide Array Sequence Analysis
Photosynthesis
Plant Proteins - genetics
Plants
Plastids
Proteins
RNA
Seeds
Seeds - physiology
Starch - metabolism
Starches
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Summary:We have used cDNA microarrays to examine changes in gene expression during Arabidopsis seed development and to compare wild-type and mutant wrinkled1 (wri1) seeds that have an 80% reduction in oil. Between 5 and 13 days after flowering, a period preceding and including the major accumulation of storage oils and proteins, ∼35% of the genes represented on the array changed at least twofold, but a larger fraction (65%) showed little or no change in expression. Genes whose expression changed most tended to be expressed more in seeds than in other tissues. Genes related to the biosynthesis of storage components showed several distinct temporal expression patterns. For example, a number of genes encoding core fatty acid synthesis enzymes displayed a bell-shaped pattern of expression between 5 and 13 days after flowering. By contrast, the expression of storage proteins, oleosins, and other known abscisic acid-regulated genes increased later and remained high. Genes for photosynthetic proteins followed a pattern very similar to that of fatty acid synthesis proteins, implicating a role in CO2 refixation and the supply of cofactors for oil synthesis. Expression profiles of key carbon transporters and glycolytic enzymes reflected shifts in flux from cytosolic to plastid metabolism. Despite major changes in metabolism between wri1 and wild-type seeds,
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1105/tpc.000877