A two-component Mn2+-sensing system negatively regulates expression of the mntCAB operon in Synechocystis

Mn is an essential component of the oxygen-evolving machinery of photosynthesis and is an essential cofactor of several important enzymes, such as Mn-superoxide dismutase and Mn-catalase. The availability of Mn in the environment varies, and little is known about the mechanisms for maintaining cytop...

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Bibliographic Details
Published in:The Plant cell 2002-11, Vol.14 (11), p.2901-2913
Main Authors: Yamaguchi, Katsushi, Suzuki, Iwane, Yamamoto, Hiroshi, Lyukevich, Alexander, Bodrova, Irina, Los, Dmitry A, Piven, Irina, Zinchenko, Vladislav, Kanehisa, Minoru, Murata, Norio
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ATP-Binding Cassette Transporters - genetics
Bacterial Proteins
Cyanobacteria - genetics
Cyanobacteria - physiology
Gene Expression Regulation, Bacterial - genetics
Gene Expression Regulation, Bacterial - physiology
Genetic Complementation Test
Manganese - pharmacology
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Operon
Photosystem II Protein Complex
Proteins - genetics
Proteins - physiology
Sequence Homology, Amino Acid
Signal Transduction - drug effects
Signal Transduction - genetics
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Summary:Mn is an essential component of the oxygen-evolving machinery of photosynthesis and is an essential cofactor of several important enzymes, such as Mn-superoxide dismutase and Mn-catalase. The availability of Mn in the environment varies, and little is known about the mechanisms for maintaining cytoplasmic Mn(2+) ion homeostasis. Using a DNA microarray, we screened knockout libraries of His kinases and response regulators of Synechocystis sp PCC 6803 to identify possible participants in this process. We identified a His kinase, ManS, which might sense the extracellular concentration of Mn(2+) ions, and a response regulator, ManR, which might regulate the expression of the mntCAB operon for the ABC-type transporter of Mn(2+) ions. Furthermore, analysis with the DNA microarray and by reverse transcription PCR suggested that ManS produces a signal that activates ManR, which represses the expression of the mntCAB operon. At low concentrations of Mn(2+) ions, ManS does not generate a signal, with resulting inactivation of ManR and subsequent expression of the mntCAB operon.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.006262