AXR1-ECR1-Dependent Conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 Is Required for Auxin Response

Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we sho...

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Bibliographic Details
Published in:The Plant cell 2002-02, Vol.14 (2), p.421-433
Main Authors: del Pozo, Juan C., Dharmasiri, Sunethra, Hellmann, Hanjo, Walker, Loni, Gray, William M., Estelle, Mark
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - pharmacology
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Auxins
Cell Nucleus - metabolism
Developmental biology
Embryos
Gels
Gene Expression Regulation, Plant - drug effects
Genetic mutation
Growth Substances
Indoleacetic Acids - pharmacology
Meristem - genetics
Meristem - metabolism
Mutation
Peptide Synthases - genetics
Plant cells
Plant Proteins - genetics
Plant roots
Plants
RNA
Seedlings
SKP Cullin F-Box Protein Ligases
Ubiquitins
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Summary:Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-box (SCF) subunit AtCUL1 is modified by RUB1 in vivo. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in vivo. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.010282