Anti‐endothelial cell antibodies in sera of patients with autoimmune diseases: comparison between ELISA and FACS analysis

In some patients suffering from rheumatoid arthritis (RA), vasculitis is a clear clinical manifestation, mentioned as rheumatoid vasculitis (RV). Autoantibodies directed against endothelial cells (AEA) have been implicated in the pathogenesis of this disorder, and it has been suggested in a number o...

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Bibliographic Details
Published in:Clinical and experimental immunology 1994-06, Vol.96 (3), p.444-449
Main Authors: WESTPHAL, J. R., BOERBOOMS, A. M. TH, SCHALKWUK, C. J. M., KWAST, H., WEIJERT, M., JACOBS, C., VIERWINDEN, G., RUITER, D. J., PUTTE, I. B. A., WAAL, R. M. W.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Arthritis, Rheumatoid - complications
Arthritis, Rheumatoid - immunology
Autoantibodies - blood
Autoimmune Diseases - immunology
Biological and medical sciences
Cell Separation
Diseases of the osteoarticular system
ELISA
endothelium
Endothelium, Vascular - immunology
Enzyme-Linked Immunosorbent Assay
FACS
Female
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunoglobulin G - blood
Inflammatory joint diseases
Male
Medical sciences
Middle Aged
rheumatoid arthritis
vasculitis
Vasculitis - etiology
Vasculitis - immunology
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Summary:In some patients suffering from rheumatoid arthritis (RA), vasculitis is a clear clinical manifestation, mentioned as rheumatoid vasculitis (RV). Autoantibodies directed against endothelial cells (AEA) have been implicated in the pathogenesis of this disorder, and it has been suggested in a number of studies that testing for AEA should be included in diagnosing RV. To test this hypothesis, we have evaluated the presence of AEA in sera of patients suffering from various autoimmune diseases, employing an ELISA with fixed cultured endothelial cells (EC). In ail the groups of patients ELISA‐positive sera were present. A significant difference in percentage of positivity was found between the RA and RV group (P
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1994.tb06049.x