Dichotomy of the human T cell response to Leishmania antigens. II: Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients

The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) pr...

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Bibliographic Details
Published in:Clinical and experimental immunology 1994-06, Vol.96 (3), p.416-421
Main Authors: KURTZHALS, J. A. L, HEY, A. S, KHARAZMI, A, JARDIM, A, KEMP, M, SCHAEFER, K.-U, ODERA, E. O, CHRISTENSEN, C. B. V, GITHURE, J. I, OLAFSON, R. W, THEANDER, T. G
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - blood
Antigens, Protozoan
Biological and medical sciences
Glycosphingolipids - immunology
Human protozoal diseases
Humans
In Vitro Techniques
Infection and Immunity
Infectious diseases
Interferon-gamma - biosynthesis
Interleukin-4 - biosynthesis
Leishmania donovani
Leishmania donovani - immunology
Leishmaniasis, Visceral - immunology
Leshmaniasis
Lymphocyte Activation
Medical sciences
Metalloendopeptidases - immunology
Parasitic diseases
Protozoal diseases
Protozoan Proteins - immunology
T-Lymphocyte Subsets - immunology
T-Lymphocytes - immunology
T-Lymphocytes, Helper-Inducer - immunology
Tropical medicine
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Summary:The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC from cured patients, while cells from non-exposed donors gave weak responses. A similar pattern was induced by lipophosphoglycan-associated protein (LPGAP). By contrast, the major surface protease of Leishmania, gp63, induced only a weak proliferative response without IFN-gamma production in five of 17 samples from cured patients. Four of the five responding cultures produced IL-4, i.e. the response to this antigen was of the Th2 type. Furthermore, sera from acutely ill visceral leishmaniasis patients contained high levels of IgG antibodies to gp63. The Th2-like response to gp63 in patients cured of visceral leishmaniasis differs from the Th1-like response to the same antigen observed in patients cured of cutaneous leishmaniasis.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1994.tb06044.x