Increased CD45RO expression on T lymphocytes in mediastinal lymph node and pulmonary lesions of patients with pulmonary sarcoidosis

SUMMARY Sarcoidosis is characterized by a cell‐mediated response mediated by the activation of CD4′ T lymphocytes in an environment lacking adequate numbers of regulatory CD8 T lymphocytes. Immunohistological studies on frozen tissues have shown that sarcoid lesions have activated CD4 heiper/inducer...

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Published in:Clinical and experimental immunology 1994-03, Vol.95 (3), p.509-513
Main Authors: FAZEL, S. B., HOWIE, S. E. M., KRAJEWSKI, A. S., LAMB, D.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
CD45 histopathology
Female
Humans
Immunohistochemistry
immunology of sarcoidosis
Leukocyte Common Antigens - analysis
Leukocyte Common Antigens - classification
Lymph Nodes - immunology
Male
Mediastinum - anatomy & histology
Mediastinum - pathology
Medical sciences
Sarcoidosis, Pulmonary - immunology
Sarcoidosis, Pulmonary - pathology
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
T lymphocyte subsets
T-Lymphocytes - immunology
Tuberculosis - immunology
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Summary:SUMMARY Sarcoidosis is characterized by a cell‐mediated response mediated by the activation of CD4′ T lymphocytes in an environment lacking adequate numbers of regulatory CD8 T lymphocytes. Immunohistological studies on frozen tissues have shown that sarcoid lesions have activated CD4 heiper/inducer T lymphocytes at the centre of granulomata, whereas lymphocytes at the periphery are mainly CD8 suppressor/cytotoxic cells. In this study we investigated the immunohistological distribution of CD45 isoforms of T cells in 29 paraffin‐embedded sarcoid lesions in mediastinal and open lung biopsies. Ten of these were assessed quantitatively, with single‐staining of serial sections demonstrating a predominance of CD45RO memory T lymphocytes in granulomata and intergranulomalous areas. Ratios of CD45RO;CD45RA T lymphocytes (or the ratio oi memory to naive T cells) were 420:1 for granulomata and 17‐9:l for intergranulomatous areas of sarcoid lesions counted. This finding is compatible with the hypothesis that nearly all the lymphocytes present in sarcoid lesions have been previously activated, and selectively home to sarcoid lesions.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1994.tb07027.x