IgM anti‐idiotypes that block anti‐HLA antibodies: naturally occurring or immune antibodies?

SUMMARY Using dithiothreitol (DTT) technique, IgM anti‐HLA anti‐idiotypic antibodies were detected in a multiparous multitransfused woman. These antibodies were able to inhibit the binding of specific IgG anti‐HLA antibodies on their corresponding antigen. The recognized determinants were crossrcact...

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Bibliographic Details
Published in:Clinical and experimental immunology 1991-01, Vol.83 (1), p.116-120
Main Authors: URLACHER, A., TONGIO, M. M., PASQUALI, J. L.
Format: Article
Language:English
Subjects:
Adult
Antibodies, Anti-Idiotypic - analysis
Antibodies, Anti-Idiotypic - immunology
anti‐HLA antibodies
Blood Transfusion
Cytotoxicity, Immunologic - immunology
Dithiothreitol
DTT cytotoxicity technique
Epitopes - immunology
Female
HLA Antigens - immunology
Humans
IgM anti‐idiotypic antibodies
Immunoglobulin G - immunology
Immunoglobulin M - analysis
Immunoglobulin M - immunology
Kidney Diseases - immunology
Renal Dialysis
Time Factors
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Summary:SUMMARY Using dithiothreitol (DTT) technique, IgM anti‐HLA anti‐idiotypic antibodies were detected in a multiparous multitransfused woman. These antibodies were able to inhibit the binding of specific IgG anti‐HLA antibodies on their corresponding antigen. The recognized determinants were crossrcactive determinants since they were partially found on anti‐HLA antibodies from unrelated individuals. By studying the patient's sera over a period of 2 years, no IgM‐IgG switch was observed bui the presence of these antibodies was stable in time, despite the disappearance of the idiotypes (anti‐HLA antibodies). However, when looking at the patient's earlier serum, it was shown that these IgM anti‐idiotypic antibodies were absent from the first available serum. Thus, these anti‐idiotypic antibodies seem to behave both like natural and immune antibodies. The incidence of such antibodies in pretransplant patients is discussed.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1991.tb05599.x