Characteristics of suppressor factors generated from human mononuclear cells using concanavalin A and C-reactive protein

Lymphocytes from normal human donors were cultured in Marbrook flasks in the presence of C-reactive protein (CRP), concanavalin A (Con A) or serum amyloid A protein low molecular weight component (SAAL) and the culture supernatants were tested for their ability to suppress the pokeweed mitogen (PWM)...

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Bibliographic Details
Published in:Clinical and experimental immunology 1983-08, Vol.53 (2), p.451-457
Main Authors: Wangel, A G, Kontiainen, S
Format: Article
Language:English
Subjects:
Amyloid - pharmacology
C-Reactive Protein - pharmacology
Cell Survival
Cells, Cultured
Concanavalin A - pharmacology
DNA - biosynthesis
Hemolytic Plaque Technique
Humans
Immunoglobulins - analysis
Lymphocytes - immunology
Lymphocytes - metabolism
Lymphokines - immunology
Molecular Weight
Pokeweed Mitogens - pharmacology
RNA - biosynthesis
Serum Amyloid A Protein - pharmacology
Suppressor Factors, Immunologic
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Summary:Lymphocytes from normal human donors were cultured in Marbrook flasks in the presence of C-reactive protein (CRP), concanavalin A (Con A) or serum amyloid A protein low molecular weight component (SAAL) and the culture supernatants were tested for their ability to suppress the pokeweed mitogen (PWM) driven response of normal human lymphocytes. The supernatants from CRP and Con A stimulated lymphocytes, when added to cultures at initiation at a final concentration of 5%, suppressed the plaque forming cell (PFC) response in the three major immunoglobulin (Ig) classes. The suppressive effect could be removed by absorption of the culture supernatants with lentil lectin and with antisera to antigen specific helper and suppressor factors and could be recovered in the eluate. The results indicate that CRP stimulated cells exert their suppressive effect through a soluble mediator(s) which is similar to Con A generated suppressor factor(s) in respect to its time of action, its effect on the Ig classes M, G and A, its lack of cytoxicity and some structural aspects.
ISSN:0009-9104
1365-2249